FastQCFastQC Report
Mon 25 Sept 2023
SRR2724738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37715021
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT8510132.2564298718009463No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC7665832.0325668120402214No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7409761.964670787270674No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA6863651.8198717163646814No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5401871.4322860909980668No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4734321.2552876478578656Illumina PCR Primer Index 6 (96% over 29bp)
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3101120.8222506358938524No Hit
ATAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTC2745230.7278877028863381No Hit
CCTCAATCCTATTTCCTATAACAACTGTGCAGCCCAACTCTTTTTCTTTA2444930.6482642552419631No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT2367560.6277498824672535No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT2345890.6220041611537217No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA2118110.5616091265069162No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT1706590.45249610228243015No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1650160.43753389398881687No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1637110.4340737341760993No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1211490.3212221464758034No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA1150290.304995190112714No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA1138030.3017444959131801No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAACTGTAGGCACCATCAATATCT1056240.2800581762900251No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC1047180.277655950397058No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1046680.27752337722415693No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC964960.2558556178452081No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT916140.24291117324314895No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT905620.24012183368531068No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCCTGTAGGCACCATCAAT896380.23767188145009918No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT885270.23472610554823767No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT861840.22851372666609412No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA838790.2224021033953554No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATA832620.22076615044175635No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT798470.21171140273261416No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCTGTAGGCACCATCAATATC780940.20706338729070312No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA765940.20308619210367135No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATAT750620.199024150085983No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT746600.19795826177585849No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA724640.19213564802204405No Hit
ATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCT710160.18829632893482942No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT704420.18677438890992532No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT657960.17445568968395908No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT657460.17432311651105803No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC642910.17046523717963727No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATAT640110.16972282741139136No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT627520.16638463491774272No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATAT614970.1630570482779262No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA604080.16016960457214116No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA601040.15936355968090274No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT580590.1539413169092495No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACTGTAGGCACCATCAATATCTC578730.15344814470605758No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT578310.15333678324082067No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA576870.15295497250286563No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT565350.14990048659922528No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT561760.1489486112177957No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCTGTAGGCACCATCA556080.1474425799736397No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT547510.14517027579011557No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT533620.14148739304692418No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATATC527570.1398832576548214No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAATATC513090.1360439385676068No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC510860.13545266221646807No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCG510600.13538372416655953No Hit
AGGATAGCTGGCGCTCTCGCTCTTTTTTCTGTAGGCACCATCAATATCTC494850.1312076692201762No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT485990.12885847259636948No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGACTGTAGGCACCATCAATAT470860.1248468083843835No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAATA465590.12344948714200635No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAT458210.12149270710998675No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT456160.12094915710109244No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT455280.12071582831678657No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCTC435110.11536782652195791No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA424880.1126553794044023No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT414850.1099959615560071No Hit
AAGGAGGATTTAGTAGTAAATTAAGAATCTGTAGGCACCATCAATATCTC409220.10850318762914118No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCCTGTAGGCACCATCAAT401350.10641648588767855No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATATC396090.10502181610875944No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA389630.10330897071487777No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATA382240.10134953921940014No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGCTGTAGGCACCATCAAT378460.10034728603226814No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATCT378070.10024387895740533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)