FastQCFastQC Report
Mon 25 Sept 2023
SRR2724739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46205815
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA19798584.284867608113827No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA17105193.701956128249226Illumina PCR Primer Index 6 (96% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA15094423.26677930039758No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT6493831.4054140155302963No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5882561.273121142869139No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC5472281.1843271241942166No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT5074251.098184286977732No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA4739281.025689082640356No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3412620.7385693770362021No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2174430.4705966121363729No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2112330.4571567453144155No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1712880.3707065874717284No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC1614670.3494516869792254No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1520860.3291490475819981No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1395860.30209617555712415No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT1240210.2684099393117511No Hit
ATAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTC1199070.2595062980709246No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1175090.2543164751016728No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA1144550.2477069174085556No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1051740.2276207009875272No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT1041930.22549759159101512No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1030990.22312992423139813No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA1006760.2178859955180966No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT1006280.2177821124895211No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT999020.2162108816823164No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT909660.19687132452917452No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATC884340.19139149477181605No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT862510.18666698120139208No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT793290.17168618278889788No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT721560.15616216270614422No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT705640.1527167089250563No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA698080.15108055122499192No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT695800.15058710683925822No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT692720.14992052407256531No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC690210.14937730240230587No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT681950.1475896486189022No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT663030.14349492590921725No Hit
AGGATAGCTGGCGCTCTCGCTCTTTTTTCTGTAGGCACCATCAATATCTC637570.13798479693519095No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA621180.1344376243552895No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT612450.13254825177307228No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATAT587370.12712036353000158No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT570240.12341303794771286No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC565500.12238719304052963No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC559290.1210432063583339No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT554950.12010393064163027No Hit
ACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA550600.11916249069516467Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA541390.11716923508437195Illumina PCR Primer Index 6 (96% over 29bp)
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT523770.11335586224374572No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT512770.11097520950555684No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT507170.10976324083884247No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA505070.1093087525888246Illumina PCR Primer Index 6 (100% over 28bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA502980.10885642856856868No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT496500.10745400768279922No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAACTGTAGGCACCATCAATATCT489810.10600613797202797No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT487040.10540664632795677No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA482810.10449117713863504No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACTGTAGGCACCATCAATATCTC472190.10219276513140174No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA465340.10071026774443866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)