FastQCFastQC Report
Tue 26 Sept 2023
SRR2724740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42143560
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA17334844.11328326320795Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13558243.2171558359094488No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13106063.1098606762219423No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT5463801.2964732927166096No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC4976081.1807450533367376No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA4568361.0839995482109248No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT3949220.9370874221351969No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3332200.7906783385172016No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3224890.7652153733571629No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC2779320.6594886620873984No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2575490.6111230280498372No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT2250170.5339297392057054No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1865340.4426156689183353No Hit
ACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1734490.4115670342040397Illumina PCR Primer Index 6 (96% over 29bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1699010.4031481915623645No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1631580.3871481194279743No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1133950.2690683938423807No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA1024170.2430193367622479No Hit
ATAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTC985010.23372728834488593No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT930980.22090682419805066No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT926150.21976074161746184No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA906300.21505065068067342No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC896250.2126659446900072No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT873450.2072558654276003No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT838020.19884888699483386No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA816730.19379710684147233Illumina PCR Primer Index 6 (96% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT788770.18716264121967863No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT779970.18507454045173213No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT726810.17246051353990977No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT712250.169005655905671No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA706610.16766737314075983No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA692920.16441895274153392No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA669920.1589614166434919No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT667480.1583824432487431No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT641100.1521228866284671No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT627760.1489575156916027No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT620410.147213476982011No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA607380.1441216641403811Illumina PCR Primer Index 6 (100% over 28bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA605280.14362336736621206No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT593620.14085663384868294No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA585870.13901768146782092No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT566640.13445470672150145No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT524410.12443419587713994No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATAT522830.12405928687562227No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT520840.12358709136105256No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT499230.11845938027067482No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA466440.11067883206829228No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT463320.10993850543238397No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA449940.10676364312839258No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC448080.10632229455698569No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT447110.10609212890415523No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA443800.10530671827439352No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT437520.10381657363544988No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT431980.10250201928835628No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATAT426250.10114238094740928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)