FastQCFastQC Report
Tue 26 Sept 2023
SRR2724741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46710179
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA19223254.115430600255246No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13956362.987862666936044No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12945852.771526523158903Illumina PCR Primer Index 6 (96% over 29bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC11360392.432101576831893No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT9912602.1221498637374094No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT8866091.8981066204006625No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA4049130.8668624455496092No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4040130.8649356706597078No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT3012880.64501572558735No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2974570.6368140871393364No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2357920.5047978942662583No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2258200.4834492284861508No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT1417170.3033963967468418No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1388760.29731421067771974No Hit
ATAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTC1359930.2911421084470689No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1286300.27537894898668663No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1260930.26994758465815344No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1235930.26459543218620507No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC1229640.26324883062426285No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT1200010.25690545951450966No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA1085100.23230482589244628No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1010200.216269777086489No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA1003750.2148889217487263No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA1002140.21454424312953285No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT957690.20502811603440868No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA950350.20345672406864465No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA943480.20198595256935323No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC939300.20109107267604348No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCCTGTAGGCACCATCAAT937690.20074639405685No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT890490.1906415301898115No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC888330.19017910421623518No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT870940.18645614695674792No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT860110.1841375945058999No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT856940.18345894157245682No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA856560.1833775888548832No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT856370.1833369124960964No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA855270.18310141778733066No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCCTGTAGGCAC834920.17874476567516473No Hit
AGGATAGCTGGCGCTCTCGCTCTTTTTTCTGTAGGCACCATCAATATCTC823040.17620142282049486No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA783060.16764226058735507No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT766240.1640413324042282No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC698590.14955840781513596No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATC687760.14723985536428794No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCTGTAGGCACCATCA685960.14685450038630768No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT666360.14265841284830016No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT657190.1406952433215895No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATAT656580.14056465080127395No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT642840.13762310780269113No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC641900.1374218668697459No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA623420.13346555576248167No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT610420.13068243647706854No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT598970.1282311506449162No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA596670.12773875261749693No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG584850.12520825492875975No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAACTGTAGGCACCATCAATATCT567120.12141250839565398No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT563010.12053261452926567No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT556270.11908967422282839No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATAT541760.11598328492810957No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA541600.1159490311522891No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT533260.11416355308764713No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT521180.11157739301320167No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC514060.1100530999891908No Hit
GTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT503010.10768744859658963No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT500610.10717364195928257No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATCT487700.10440979042276846No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA473860.10144683881429784No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC471560.10095444078687858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)