FastQCFastQC Report
Tue 26 Sept 2023
SRR2724743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40000695
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34218898.554573864279108Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13580693.3951135099027656No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8933212.233263697043264No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT6285731.5714051968347051No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC5422581.3556214460773743No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT4557131.1392627053104951No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3423660.8559001287352631No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2696480.6741082873685069No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT2432210.6080419352713746No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA2380540.5951246597090376No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1994580.49863633619365866No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1612690.40316549499952437No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1430170.3575362878069993No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1393100.34826894882701415No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT1233970.30848714003594185No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA1188920.29722483571847935No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCCTGTAGGCACCATCAAT1094100.2735202475856982No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT1083750.27093279254272956No Hit
ATAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTC1018430.25460307627154977No Hit
ACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1004360.2510856373870504Illumina PCR Primer Index 6 (96% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT985300.2463207201774869No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC967230.24180329866768566No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT944860.23621089583568486No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA915770.2289385221931769Illumina PCR Primer Index 6 (96% over 29bp)
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA872730.21817870914492862No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT849060.21226131195970468No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT831450.20785888845181316No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC820980.20524143393008545No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA797860.19946153435584058No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA777640.19440662218493954No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATAT777340.1943316234880419No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT769330.19232915828087488No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT765750.19143417383122968No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA744960.18623676413622314No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA738770.18468929102356846Illumina PCR Primer Index 6 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT726830.18170434288704232No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAACTGTAGGCACCATCAATATCT726210.18154934558012054No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAATA673270.16831457553425008No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT654320.1635771578468824No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA642160.16053721066596466No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT641930.16047971166500982No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATC626610.15664977821010362No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT602200.15054738423919883Illumina PCR Primer Index 6 (96% over 29bp)
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT575630.14390499965063105No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC551290.1378201053756691No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT545890.13647012883151155No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT543280.13581764016850206No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT542160.13553764503341756No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT518810.1297002464582178No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT518020.12950274988972066No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT517180.12929275353840727No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC516520.12912775640523247No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC516220.12905275770833483Illumina PCR Primer Index 6 (96% over 29bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA501630.12540532108254618No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCCTGTAGGCAC488630.12215537755031507No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA484490.12112039553312762No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCTGTAGGCACCATCAATATC473850.11846044174982459No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA461910.11547549361329848No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT455250.11381052254217083No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATAT454540.11363302562617975No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCTGTAGGCACCATCAATA447060.11176305811686521No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGACTGTAGGCACCATCAATAT430110.10752563174214848No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCTGTAGGCACCATCA429280.10731813534739834No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA422190.10554566614405074No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT417300.1043231873846192No Hit
AAATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCA414650.10366069889535669No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC414380.10359320006814882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)