FastQCFastQC Report
Tue 26 Sept 2023
SRR2724744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51618253
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA20432523.958390455407315No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA14228402.7564667870491473No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10335912.0023750125754933Illumina PCR Primer Index 6 (96% over 29bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC9807261.899959690615643No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT9587201.8573274845237402No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT8366951.620928550216529No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4147460.8034870920563701No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA3941980.7636794681912229No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3121310.6046911351300479No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2264920.4387827693432399No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2250690.4360259925883195No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1811320.350906877844161No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA1811210.3508855675530127No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1544040.29912674495202307No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1484210.28753588386650747No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1469350.2846570572622828No Hit
ATAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTC1297140.25129482781991863No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA1247280.24163545403212308No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT1245050.24120343631156985No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1180040.22860905424288577No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC1173660.22737305735628055No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT1167530.22618549294955798No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC1159410.2246124060029695No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT1153560.22347908597371555No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATAT1041390.20174840089996846No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1041030.20167865812893748No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT1025460.19866228328184604No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT992950.19236412359790633No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCCTGTAGGCACCATCAAT967330.18740076305953243No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT966370.18721478233678307No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATC921310.17848531216273436No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT916600.17757284424174527No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT892690.17294076186576868No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA873950.1693102631737653No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG829610.16072027854177862No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA825240.1598736787934299No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT813310.1575624808534299No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT780250.15115776971374834No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT720650.13961146650972475No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT720200.139524288045936No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAATA711490.1378369004468245No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC707210.13700773639123356No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAT702090.13601583920323687No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT688080.13330168303061324No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT671800.13014775994065508No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA671740.13013613614548328No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT655310.12695315356759557No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATAT653400.12658312942129213No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGACTGTAGGCACCATCAATAT648340.1256028560284673No Hit
AGGATAGCTGGCGCTCTCGCTCTTTTTTCTGTAGGCACCATCAATATCTC636160.12324322560858463No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC633130.1226562239524069No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA631530.12234625608115796No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT627380.12154227691510598No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATA616050.1193473169268243No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAACTGTAGGCACCATCAATATCT589660.11423478435041184No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC577050.11179185006513102No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT569290.1102885058895736No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT555280.10757434971694993No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC552040.1069466647776708No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCAA546990.10596832868404128No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCCTGTAGGCAC537360.10410270955896163No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC526500.10199880263285935No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)