FastQCFastQC Report
Sat 23 Sept 2023
SRR2732970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2732970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378053973
Total Bases28.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA105886102.8008196596838832Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG73502901.944243553816587No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGAT21768180.5757955623971184No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA20974140.5547922121691339No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAGA18427200.48742246652702154No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA16278330.43058216981097563No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA15725300.41595383524775176No Hit
CGCGACCTCAGATCAGACGTGGCGCTGTAGGCACCATCAATAGATCGGAA15592870.41245089626395753No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG15169990.4012651918354525No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC14785980.3911076474786842No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG13803040.36510765620230634No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG13688160.36206893664889483No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA13456730.35594732395524914No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13344040.3529665326384495No Hit
ACGCGACCTCAGATCAGACGTGGCGCTGTAGGCACCATCAATAGATCGGA13220420.3496966291635824No Hit
AACCATCCTCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC12721390.33649666207845935No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG12689590.33565551234135554No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG12670420.33514844188662973No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT12284770.3249475174805265No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC11529100.30495910169948137No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA11510640.3044708116319677No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG11072060.2928698225848297No Hit
ATCCCACTTCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC10957950.289851470493606No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10463210.27676497926924315No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT9278190.2454197194748169No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC9002090.23811652946178669No Hit
ATCCCACTCCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC8910820.23570232391130036No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA7927280.20968646188516576No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG7466610.19750116473448623No Hit
ATCCCACCGCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC7342010.19420533903501655No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAG7061150.18677624107391672No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG7056180.18664477836343227No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC6904170.18262392391257848No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC6873480.18181213506252453No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG6572370.17384739929713686No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA6457570.17081079584369294No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC6310520.16692113959082766No Hit
CCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6298710.16660875033311712No Hit
CGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAGAT6285440.16625774225099862No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG6162190.16299762573848153No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA6100730.16137193193840604No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGAT6054180.16014062626978398No Hit
ACCCCACTCCTGGTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC5845040.15460861192959874No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA5788630.1531164969399753No Hit
AAATGGATTTTTGGAGCAGGGACTGTAGGCACCATCAATAGATCGGAAGA5675950.1501359701356716No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGATC5660940.14973893687925877No Hit
CGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGATC5524300.14612463813467186No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5259440.13911876016708336No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG5217210.1380017238967093No Hit
AACCATCCTCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5212290.1378715837487046No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG5199150.13752401432903338No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC5174710.1368775457889448No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA5135700.13584568254226492No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC4976850.13164390154418507No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG4770250.12617907337796977No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTCTGTAGGCACCATCAATAGA4722180.1249075618099641No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA4508150.11924620085926196Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC4487650.11870395024257555No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC4437610.1173803297128688No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC4316180.11416835447461361No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA4072220.10771530762354929No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4054760.10725346880563003Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG4002260.10586477820192092No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3925360.103830677108107Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3908060.10337307049012284No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3874490.10248510204123684No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3874050.1024734634914153No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC3796470.10042137554787713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)