FastQCFastQC Report
Sat 23 Sept 2023
SRR2733100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2733100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388812515
Total Bases29.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG79229482.0377296754452465No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG38189700.9822137541020253No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC33433000.8598745850554734No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG31632220.8135597178501314No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA28851220.7420342423905774No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT28634470.7364595761533036No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG28547140.7342135064762512No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT23237140.5976438284143194No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT21753660.5594897067549381No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT21670070.557339827397274No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG17900490.4603887300284046No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA17365080.4466183399472108No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATC16094660.41394398017255185No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15145450.3895309285504866Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC14759440.379603007377476No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGAT13830580.3557133442579645No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAGA13533520.3480731580875168No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA13483070.34677561754924474No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA12821830.3297689633267077No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC11424920.29384136464846045No Hit
ACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC10489510.26978323987333586No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG9908480.2548395336502993No Hit
ATCCCACCGCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC9324400.23981738345022152No Hit
ATCCCACTTCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC7998720.20572177312759596No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA7476040.19227879020303654No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA7363630.18938767956067465No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA7336940.1887012304632221No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA7082020.18214485714277998No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC7064800.18170197016420625No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCG6311390.1623247646748202No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG6299300.16201381789369615No Hit
ATCCCACTCCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC6215720.1598641957293993No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC5694470.14645799145637067No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG5590810.1437919250104385No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC5551590.14278321262369859No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGAT5330660.13710103955887326No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGAT5122450.1317460164573149No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT4999590.12858613874607405No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC4970110.12782793269913137No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC4845150.12461404438074736No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA4816020.12386484010166185No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAAT4793310.12328075396441393No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA4685730.12051386771848123No Hit
ACCCCACTCCTGGTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4669320.12009181340266273No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA4650960.1196196063802113No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATC4567490.11747281334295528No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG4521760.11629666807407164No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4482180.11527869672610719No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCCTGTAGGCACCATCAATAGAT4351640.11192129450874286No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAG4262810.10963664582658818No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA4142520.10654286681075582No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC4066990.10460028530717433No Hit
GCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG4028560.10361189119645493No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG3980320.10237119039236686No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA3913760.10065931133929679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)