FastQCFastQC Report
Fri 29 Sept 2023
SRR2733385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2733385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223555868
Total Bases11.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA138637806.201483380431776No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG105111064.701780406855614No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA73832773.302654081976502No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA48814002.1835257753108945No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC38265201.7116616236617865RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA31971931.4301539157093386No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA25852601.156426813184792RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG25718311.1504198136279742No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGG19575630.8756482294618185Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA19113830.854991200678302No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAG18954600.8478685963188406No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA18852060.8432818234053243RNA PCR Primer, Index 1 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGCC18334420.82012698499151No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAA16983640.7597045048265072No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA16433640.7351021535252208RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA15611460.6983247695381452No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGA15031960.6724028375761535RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA12839070.574311473676012No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG12729470.5694088960348829No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG11549850.5166426675948403Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGC9164930.40996150456672426No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGCC8798440.3935678396059817No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGA8635160.386264072477847RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC8418670.3765801396901825No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA6205900.27759951261936905RNA PCR Primer, Index 1 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAACT6202760.27745905555921263RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA6190150.27689499074119583RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG5424890.2426637264560642No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG5233100.23408466289956656Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG5179920.23170583918647128No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCA4996100.22348328606610315No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA4420450.19773357056322047RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA3395560.15188865451744707RNA PCR Primer, Index 1 (100% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTGGAATTCTCGGGTGCC3050110.1364361413228482No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGA2875190.12861169897808272RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGATGGAATTCTCGGGTGCCAAGGAAC2838590.12697452432785167RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGGGCCAAG2791400.12486364258620132No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2702870.12090355865765061No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC2652680.1186584822725387No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2632900.1177736922566488No Hit
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA2527630.11306480221758258RNA PCR Primer, Index 1 (100% over 22bp)
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA2449710.10957932001140762No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTGCCAAG2405070.10758250371669957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)