FastQCFastQC Report
Sat 1 Jul 2023
SRR2818787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2818787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116384315
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC57190514.913936212108994RNA PCR Primer, Index 6 (100% over 50bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG30129832.5888222137149666No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG15485521.330550426833719Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC11228280.9647588680656839RNA PCR Primer, Index 1 (100% over 31bp)
CGCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAG9230830.7931335077239575RNA PCR Primer, Index 1 (100% over 29bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG7624310.6550977251530844Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCC6327900.5437072856423996RNA PCR Primer, Index 1 (100% over 27bp)
TACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGA5849510.5026029495469385RNA PCR Primer, Index 1 (100% over 22bp)
CCGCGAGGGGGGCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5634160.48409959709777045RNA PCR Primer, Index 1 (100% over 32bp)
CTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAAC4917440.4225174156844073RNA PCR Primer, Index 1 (100% over 24bp)
CGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4888270.4200110642056879RNA PCR Primer, Index 1 (100% over 32bp)
CGCTGAGAAGACGGTCGAACTTGACTATGGAATTCTCGGGTGCCAAGGAA4465030.3836453391507266RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC3915450.33642419942927876RNA PCR Primer, Index 1 (100% over 24bp)
GCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGA3895310.3346937256966285RNA PCR Primer, Index 1 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCTGGAATTCTCGGGTGCCAAGGAACT3720000.3196306993773173RNA PCR Primer, Index 1 (100% over 25bp)
CGCGAGGGGGGCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3497120.3004803525286032RNA PCR Primer, Index 1 (100% over 33bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGG3480570.2990583396052982Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG3300100.2835519545739475No Hit
AAGCATAGAGACGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC3270600.281017248758993RNA PCR Primer, Index 6 (100% over 35bp)
CGCTGAGAAGACGGTCGAACTTGACTTGGAATTCTCGGGTGCCAAGGAAC3087550.2652891843716226RNA PCR Primer, Index 1 (100% over 24bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGGAATTCTCGGGTGCCAA3036810.26092949036990076No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGGAATTCTCGGGTGCCAAG2887160.248071228498445No Hit
ACCCCCCTCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA2840820.24408959231319102RNA PCR Primer, Index 6 (100% over 37bp)
CGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG2786050.2393836317204771RNA PCR Primer, Index 1 (100% over 29bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG2762750.2373816437378181No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG2750210.2363041789608849Illumina Small RNA Adapter 2 (100% over 21bp)
GTACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC2709710.23282432860476085RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAA2704750.23239815433892444RNA PCR Primer, Index 1 (100% over 23bp)
CTACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGG2694110.2314839418009205Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATGGAATTCTCGGGTGCCAAGGAACT2214180.1902472854696958RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGAA2213670.19020346513187794RNA PCR Primer, Index 1 (100% over 23bp)
TACTTGGATAACTGTGGTAATTCTTGGAATTCTCGGGTGCCAAGGAACTC2211820.19004450900449943RNA PCR Primer, Index 1 (100% over 26bp)
CTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGGTGCCAAGGAACTC2171190.18655348875834343RNA PCR Primer, Index 1 (100% over 26bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA2170060.18645639663729602RNA PCR Primer, Index 1 (100% over 22bp)
TTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAACT2167800.1862622123952012RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA2110420.1813319947795371No Hit
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT2059110.1769233251061365RNA PCR Primer, Index 1 (100% over 25bp)
TAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTC2036640.17499265257522031RNA PCR Primer, Index 1 (100% over 26bp)
ATGCGTGCATTTATCAGATCAAAACCTGGAATTCTCGGGTGCCAAGGAAC2012760.17294082969857236RNA PCR Primer, Index 1 (100% over 24bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC1962690.16863870359163088RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCCATATCTCGTATGC1949320.16748992336295487RNA PCR Primer, Index 6 (98% over 50bp)
ATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAG1926190.1655025421595685RNA PCR Primer, Index 1 (100% over 29bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1878810.16143154685405847RNA PCR Primer, Index 1 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGACTATTGGAATTCTCGGGTGCCAAGGA1862060.15999234948455038RNA PCR Primer, Index 1 (100% over 22bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAG1843260.15837701154146072No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA1766370.151770451198686RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1672320.1436894653716869No Hit
CCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTGCCAAG1650740.1418352636263744No Hit
GCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1639720.14088840064058458RNA PCR Primer, Index 1 (100% over 30bp)
GATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCA1618170.13903677656220256RNA PCR Primer, Index 1 (100% over 28bp)
CGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGGGTGCCAAGGAACTC1596770.13719804081847284RNA PCR Primer, Index 1 (100% over 26bp)
CGGTCGGGCTGGGGCGCGAAGCGGGGTGGAATTCTCGGGTGCCAAGGAAC1592940.13686895867368382RNA PCR Primer, Index 1 (100% over 24bp)
TCCTCCTCGGCGCGCGGCGGCGGTGGAATTCTCGGGTGCCAAGGAACTCC1540280.13234429398841244RNA PCR Primer, Index 1 (100% over 27bp)
CCCGCCGGGCAGCTTCCGGGAAACCAAAGTGGAATTCTCGGGTGCCAAGG1518360.13046087868455472Illumina Small RNA Adapter 2 (100% over 21bp)
ATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1483420.12745875593287634RNA PCR Primer, Index 1 (100% over 29bp)
TCCTACTTGGATAACTGTGGTATGGAATTCTCGGGTGCCAAGGAACTCCA1451220.12469206009418021RNA PCR Primer, Index 1 (100% over 28bp)
AATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAAGGAA1446630.12429767705381949RNA PCR Primer, Index 1 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGA1436300.12341010040743032RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1425610.12249159175787563RNA PCR Primer, Index 6 (100% over 36bp)
GCCCGGATAGCTCAGTCGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACT1415150.12159284522145444RNA PCR Primer, Index 1 (100% over 25bp)
TATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1413080.12141498620325256RNA PCR Primer, Index 1 (100% over 29bp)
TGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAACTC1389110.11935543032581322RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC1385030.11900486762327037RNA PCR Primer, Index 1 (100% over 24bp)
GTGCATTTATCAGATCAAAACCTGGAATTCTCGGGTGCCAAGGAACTCCA1356680.11656897237398356RNA PCR Primer, Index 1 (100% over 28bp)
CTACTTGGATAACTGTGGTAATTCTTGGAATTCTCGGGTGCCAAGGAACT1328850.11417775668482474RNA PCR Primer, Index 1 (100% over 25bp)
CCTACTTGGATAACTGTGGTATGGAATTCTCGGGTGCCAAGGAACTCCAG1314080.11290868533272717RNA PCR Primer, Index 1 (100% over 29bp)
TAACCTCGGGCCGATCGCACGTGGAATTCTCGGGTGCCAAGGAACTCCAG1309010.11247305962147906RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC1300240.11171952165547393RNA PCR Primer, Index 1 (100% over 27bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1296040.11135864828520922RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGATGCGTGCATTTATCAGATGGAATTCTCGGGTGCCAAGGAACTC1254000.10774647769332148RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAA1247250.10716650263396746RNA PCR Primer, Index 1 (100% over 23bp)
CGTGCATTTATCAGATCAAAACCTGGAATTCTCGGGTGCCAAGGAACTCC1191690.10239266347875141RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph