FastQCFastQC Report
Mon 17 Jul 2023
SRR2818789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2818789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160053847
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC55817653.4874294524142244RNA PCR Primer, Index 12 (100% over 50bp)
ACGAGACCTTAACCTACTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC13723290.8574170666450772RNA PCR Primer, Index 1 (100% over 31bp)
CGAGACCTTAACCTACTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA12342210.7711286064870405RNA PCR Primer, Index 1 (100% over 32bp)
CTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCCAAGGAACTC7309790.4567081727188975RNA PCR Primer, Index 1 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC6941810.4337171602004668RNA PCR Primer, Index 1 (100% over 24bp)
TCCTCCTCGGCGCGCGGCGGCGGTGGAATTCTCGGGTGCCAAGGAACTCC6600720.41240620726848254RNA PCR Primer, Index 1 (100% over 27bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5279370.329849616173237RNA PCR Primer, Index 1 (100% over 31bp)
CGCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAG5187970.3241390380326191RNA PCR Primer, Index 1 (100% over 29bp)
AAGCATAGAGACGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT4893650.3057502266721524RNA PCR Primer, Index 12 (100% over 35bp)
TGCCGAAGTTTCCCTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4855950.3033947693865802RNA PCR Primer, Index 1 (100% over 33bp)
CTCTCTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCCAAGGA4512520.28193761565756054RNA PCR Primer, Index 1 (100% over 22bp)
CTCTCTCTCTCTCCCCCGCTCCCTGGAATTCTCGGGTGCCAAGGAACTCC4313400.2694968025354617RNA PCR Primer, Index 1 (100% over 27bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG4055720.25339722074908955Illumina Small RNA Adapter 2 (100% over 21bp)
CTCTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCCAAGGAAC3433700.2145340499063418RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGCCAAGGAACT3294210.20581885794972488RNA PCR Primer, Index 1 (100% over 25bp)
TCTCTCTCTCTCCCCCGCTCCCTGGAATTCTCGGGTGCCAAGGAACTCCA2986330.1865828317141293RNA PCR Primer, Index 1 (100% over 28bp)
CGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCC2949830.18430234919626767RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT2861910.1788091978820103RNA PCR Primer, Index 1 (100% over 25bp)
GCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGA2492060.15570134968389732RNA PCR Primer, Index 1 (100% over 22bp)
CTCCCCCGCTCCCCGTCCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCC2355750.14718484086171324RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC2303440.14391656577926554RNA PCR Primer, Index 1 (100% over 27bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG2273060.14201845457672754Illumina Small RNA Adapter 2 (100% over 21bp)
ACGAGACCTTAACCTACTAATGGAATTCTCGGGTGCCAAGGAACTCCAGT2169940.13557562287147024RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC2142610.13386807253686317RNA PCR Primer, Index 1 (100% over 24bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2101240.131283317420043RNA PCR Primer, Index 1 (100% over 30bp)
TCCTCCTCGGCGCGCGGCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTC2091870.13069788944217003RNA PCR Primer, Index 1 (100% over 26bp)
TCTCTCTCTCTCCCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCC2079370.1299169022785188RNA PCR Primer, Index 1 (100% over 27bp)
CTCTCTCTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCCAAG1989810.12432128544839038No Hit
CTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGTGCCAAGGAAC1933620.12081059195034531RNA PCR Primer, Index 1 (100% over 24bp)
CTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGCCAAGG1829100.11428028968275908Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCGAGGGGGGCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1706150.10659849994108545RNA PCR Primer, Index 1 (100% over 32bp)
TGCCGAAGTTTCCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1636200.10222809577329309RNA PCR Primer, Index 2 (100% over 34bp)
CGAGACCTTAACCTACTATGGAATTCTCGGGTGCCCAGGAACTCCAGTCA1602970.10015191949744263RNA PCR Primer, Index 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)