FastQCFastQC Report
Sun 2 Jul 2023
SRR2829326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2829326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46598471
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG15543483.3356201751769925No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA12715212.728675367910677No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT9267431.988784138432353No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3943300.8462294825081278No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3108700.6671248934326622No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA2516770.5400971203540134No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG2403660.5158237917291321No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1486990.3191070367952631No Hit
GAAAAGAAACCAACCGGGATTGCCTTAGTAACGCTGTAGGCACCATCAAT1437920.30857664836256105No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG1369160.2938207993991906No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAA1323610.28404580055856343No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1249050.2680452755627969No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT1226790.26326829479018743No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1156800.2482484886682226No Hit
GGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA998090.2141894312369176No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA996630.21387611623565933No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC940110.20174696289927624No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC919000.1972167713399867No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA882360.18935385240429883No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA864130.18544170687488867No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG832130.17857452876511765No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA827900.1776667736587323No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT820170.1760079209465907No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAA784130.1682737616004611No Hit
AAATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATC747980.16051599632957914No Hit
AAATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCG742400.1593185321466878No Hit
TATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCG742210.15927775827666105No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAATAGA718560.1542024844549084No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT713330.1530801300325927No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC712960.15300072828569847No Hit
AATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG709120.15217666691252596No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT708310.15200284146662238No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC707840.15190197978813513No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG703100.15088477903062528No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC694920.14912935662631507No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTCTGTAGGCACCATCA686550.14733316035197808No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG683260.14662712860256724No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATAGATCGGA674240.14469144277287552No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA663840.14245960988719997No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT647970.13905391874338538No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA643360.1380646158969465No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG626770.13450441324566206No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT624270.1339679149558362No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA616240.13224468244891555No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT611010.13112232802659984No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA610190.13094635658753698No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT598850.12851280034488685No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA592580.1271672626340036No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC586250.1258088489641645No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA558590.11987303188553118No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG544530.11685576550355053No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA524350.11252515130807618No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA518710.11131481116622903No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATAGAT504440.10825247892790302No Hit
TGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA502770.10789409807029934No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT489970.10514722682639092No Hit
TGTGCCCGAGTTGTAATTTGGAGAGGGCCTGTAGGCACCATCAATAGATC489250.10499271531892108No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCTGTAGGCACCATCAAT477420.10245400541146511No Hit
AAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAA467190.10025865440949769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)