FastQCFastQC Report
Sat 1 Jul 2023
SRR2829327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2829327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14274319
Total Bases713.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG7247985.077636278129976No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1605401.124677121199267No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1341050.9394843985201675No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1184030.82948265342816No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA1092600.7654305609955894No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT922150.6460203110214925No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA840220.5886235273290445No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG711750.4986227363981427No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA655980.4595525712995485No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA556420.3898049357030623No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT519400.3638702483810261No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT515520.361152080179797No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA488830.3424541654141259No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC442550.31003230346750693No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG393030.27534063096109873No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG379130.26560286343607703No Hit
AAGAGGCGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG370100.2592768173388867No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA342480.2399273828754983No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT339100.23755949408164412No Hit
AAGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATAGATCG336860.23599024233660462No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCAGCAATAGATCG335440.23499544881966No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT324580.22738738009147755No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG323690.2267638827463503No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC304850.21356535467646479No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC292570.2049624924313377No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA264210.18509464444503446No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA261010.18285285623783523No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG253500.17759165953906453No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT207480.14535194288428052No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA200240.14027989706549224No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA190740.1336245883253695No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG188650.1321604204025425No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG179900.12603053077348209No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA174210.12204435111755595No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGCCACCATCAA171400.1200757808481091No Hit
AATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG168860.11829636145864472No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT164830.11547310943520318No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA163200.11433119856716106No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA161580.11319629328726645No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA151610.10621172190421133No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT151350.1060295766123764No Hit
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT150840.10567229161685401No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATAGATCGGAA149700.10487365456803928No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA147150.10308722959042739No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC146550.10266689430157754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)