FastQCFastQC Report
Sat 1 Jul 2023
SRR2829332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2829332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47776816
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG20464544.283362039027465No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA14096902.950573349216072No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT9708392.032029509877762No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5280551.105253644361734No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4151910.8690219122178423No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA3228890.675827790617106No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG2797250.5854827161357927No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2629950.5504657321659945No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1829510.3829284061122868No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG1827330.38247211785733065No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1412650.29567688227696043No Hit
GAAAAGAAACCAACCGGGATTGCCTTAGTAACGCTGTAGGCACCATCAAT1248260.26126898033556695No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAA1214950.2542969795224529No Hit
GGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA1071300.22423009519931172No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1068600.22366496754409082No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT1067830.2235038015090834No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG1029180.21541410377786582No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA996380.20854884929962683No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA958020.20051985046471077No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC868960.18187901010398014No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA859430.17988431878758937No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT858950.17978385164888344No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT858430.17967501224861865No Hit
TATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCG844440.17674681376841855No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAATAGA840540.17593051826643283No Hit
AAATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCG829390.1735967503569095No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA793180.16601776058078044No Hit
AAATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATC789840.1653186767406183No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT758440.1587464514169383No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC741770.1552573114122967No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT738120.15449334254505365No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA711490.1489195094122639No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG709410.14848415181120483No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA708560.14830624125307973No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA706080.14778716103643239No Hit
AATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG701590.14684737467645395No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA694900.14544711393073995No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG679290.14217983885740731No Hit
ATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATC678890.14209611624181906No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC669460.14012235557932531No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC653200.13671903125566173No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC650960.13625018460836738No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA615800.12889096669815753No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAA608520.12736721509445084No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA590820.12366248935466943No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA589880.12346574120803697No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGA575900.12053963579322657No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG572320.11979031838371146No Hit
TGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA564270.11810540074499733No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGAT563830.11801330586785021No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT547200.11453253812476746No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATAGATCGGA533500.11166503854086886No Hit
TATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATC530160.11096595470070673No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTCTGTAGGCACCATCA517960.10841241492526416No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCTGTAGGCACCATCAAT509130.10656423818615288No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA507640.10625237144308652No Hit
AAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAA506110.10593213243846136No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG504730.10564328941468179No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC502530.10518281502894626No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG501220.10490862346289463No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT500730.104806063258799No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAA500440.1047453643624975No Hit
TTAACGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATA496190.10385581157187201No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG486910.10191344689022391No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATA482740.10104063862271609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)