FastQCFastQC Report
Sat 1 Jul 2023
SRR2829333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2829333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13459039
Total Bases672.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG8065505.992626962445089No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1919781.426387129125638No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1649101.2252732160149027No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1416461.052422836429852No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA858110.6375715234943594No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA850970.632266538495059No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT756160.5618231732592498No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA671980.499277845914556No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA645120.47932099758385427No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG611590.4544083719498844No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA524700.38984952788976984No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT508830.3780581956854423No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT495480.36813921112792675No Hit
AAGAGGCGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG397880.29562288956886146No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG383920.28525067800160175No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA382040.28385384721747225No Hit
AAGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATAGATCG381380.283363470452831No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCAGCAATAGATCG381020.28309599221757215No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT374820.27848942261033643No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA349390.2595950572696906No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG347770.2583914052110258No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC296850.2205580948238578No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA258340.19194535360214054No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC249680.18551101605396939No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG238280.1770408719374392No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT216110.16056867061608188No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC209950.1559918208127638No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA208520.15492933782270785No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG190100.14124336811863017No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT184510.13709002552113864No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG183790.13655506905062093No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA182380.1355074459625238No Hit
ATGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG175080.13008358174755272No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA174210.12943717601234384No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG173100.12861245145362904No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA170990.12704473179697304No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC169800.12616056763042294No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT169730.12610855797356707No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT166550.12374583356211391No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATAGATCGGAA162210.12052123483704891No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA161620.12008286772926358No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATAGATC158620.11785388243543986No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCACTAGATCG157380.11693256851399271No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA150040.111478984495104No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGCCACCATCAA149360.11097374782850396No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG149020.11072112949520392No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA147900.10988897498550973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)