FastQCFastQC Report
Sat 1 Jul 2023
SRR2927426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2927426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69699728
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length25-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCG15771802.2628208821704443No Hit
ATGTACACGGAGTCGACCCGCAACGCGA14823672.1267902221942676No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCG6887680.9881932394341625No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT6819430.9784012356547503No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC6731310.9657584316541379No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT6678130.9581285596982532No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT6605890.9477641003132753No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCC6323720.9072804416109056No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC6284760.9016907497831269No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAT5741840.823796615103003No Hit
GGGGGGGGGATGCGTGCATTTATCAGAT5638150.808919942987439No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC5430120.7790733415774592No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCC4967770.7127387928974415No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCC4745460.6808434030043847No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG4710710.6758577307503983No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC4614180.6620083223280298No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCC4536940.6509265000288093No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGC4154220.5960166731210199No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4137260.5935833781159089No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC3772720.5412818827643058No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCG3686360.5288915905095067No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCG3533270.5069273728012252No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3380430.48499902323865596No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC3303720.4739932414083452No Hit
TGGGGGGGGATGCGTGCATTTATCAGAT3136110.44994580179710314No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCC3121360.4478295812000873No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2799640.4016715818460583No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2707300.388423323545825No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC2647400.37982931583319807No Hit
AAATGGATTTTTGGAAGTAGGAGTTGGA2610130.37448209267043336No Hit
TCGGGGGGGGATGCGTGCATTTATCAGAT2579020.37001866061801564No Hit
GGGGGGGGGATGCGTGCATTTATCAGATC2538630.3642238029967635No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGC2509950.3601090093206677No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2437610.349730202677405No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG2420950.34733994944714847No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTT2371670.34026962056437293No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG2269290.32558089753234043No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2268260.3254331207720065No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC2246230.32227241977185334No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2238990.32123367827203No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2238350.32114185581900695No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2174660.3120040870173841No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2078960.2982737608387797No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG2064960.2962651446789003No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2051010.29426370214816333No Hit
GGGGGGGGGGATGCGTGCATTTATCAGA2045410.2934602556842115No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2040280.2927242413341986No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGC2016960.2893784607021709No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGG1880650.26982171293408774No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGC1726790.24774702133701296No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1669100.23947008803248127No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGT1664690.23883737394211926No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1606120.2304341847646809No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC1590380.228175926310645No Hit
TCGGGGGGGGATGCGTGCATTTATCAGA1569290.2251500895383695No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCC1542090.22124763528488947No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGG1487440.21340685863221734No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCG1465340.2102361145512648No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC1417160.20332360550962264No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1381070.1981456799946192No Hit
GGGGGGGGATGCGTGCATTTATCAGAT1368720.19637379359643986No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1367940.19626188498181799No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGC1357440.19475542286190844No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1354290.19430348422593557No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATC1333920.19138094771331102No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1307680.1876162271393656No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGG1300800.1866291357693677No Hit
CGGGGGGGGATGCGTGCATTTATCAGAT1218100.17476395316779428No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1215740.17442535787227176No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1193020.17116566078995316No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT1185930.1701484401775571No Hit
TGGGGGGGGGATGCGTGCATTTATCAGAT1144830.16425171702248248No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCC1136450.16304941677821183No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1098850.15765484766310708No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1095510.15717564923639302No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1089620.15633059572341515No Hit
TGGGGGGGGATGCGTGCATTTATCAGA1086310.1558557014741865No Hit
TTAACGCGAACTCGGCCTACAATAGTGA1076450.15444106180730002No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAT1062300.15241092475999332No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCA1041180.14938078381023237No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGG1033070.1482172211633308No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG998820.14330328520076865No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG985330.14136784005814199No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG977290.1402143204920398No Hit
GGGGGGGGGATGCGTGCATTTATCAGA975390.1399417225846276No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG975120.13990298498725848No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC974030.1397465998719536No Hit
TTAACGCGAACTCGGCCTACAATAGT960600.13781976308429783No Hit
TAAATGGATTTTTGGAAGTAGGAGTTGGA954100.1368871912957824No Hit
TTGGTCTAGGGGTATGATTCTCGCTT946370.13577814823036324No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG914090.13114685325601272No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCG901540.12934627234126367No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCC899470.1290492840947672No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG880500.12632760919813057No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGC875890.12566620059119885No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCC863820.12393448651621712No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT853800.12249689123607485No Hit
TGGCGGGCGTGGGGGTGGGGGCCGGGCCGC852150.12226016147437477No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG851520.12216977374718019No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC847570.12160305704492849No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC818130.11737922420586777No Hit
TGGGGGGGGATGCGTGCATTTATCAGATC811820.11647391220809356No Hit
GGGGGGGGATGCGTGCATTTATCAGATC808280.11596601926480976No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC794950.11405352973543885No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGT793760.1138827973618491No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC788430.11311808849526644No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT773050.11091148017105604No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT762380.10938062771206224No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG760180.10906498802979547No Hit
TTGGGGGGGATGCGTGCATTTATCAGAT760060.10904777131985366No Hit
CGGGGGGGGATGCGTGCATTTATCAGA753710.10813672041876549No Hit
ATTAAATGGATTTTTGGAAGTAGGAGT734540.10538635100555917No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG733560.10524574787436759No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC715490.10265319830229466No Hit
ATGGACACGGAGTCGACCCGCAACGCG701610.10066179885235707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)