FastQCFastQC Report
Sat 1 Jul 2023
SRR2927427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2927427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77801391
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length25-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA23786643.0573540773840406No Hit
ATGTACACGGAGTCGACCCGCAACGCG19599822.5192120279700396No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCG12585391.6176304611314727No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCC11160811.4345257657411292No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCC9640921.2391706467047614No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC9294661.194665015693614No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCC8878561.141182681425323No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAT7611650.9783436905389005No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCC7598450.9766470627755229No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCC7296670.937858553197333No Hit
GGGGGGGGGATGCGTGCATTTATCAGAT7238480.9303792524737765No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCG6762910.869253096001844No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT5676060.7295576501967683No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT5571850.716163288134527No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT5448620.7003242397041463No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCG5153230.6623570522023186No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC5093600.6546926648136664No Hit
TCGGGGGGGGATGCGTGCATTTATCAGAT4250700.5463526995294982No Hit
TGGGGGGGGATGCGTGCATTTATCAGAT4193010.5389376650090999No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4143800.5326125852942655No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG4045120.5199290074389543No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC3939480.5063508440356805No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3825660.4917212855487378No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG3756230.48279728057818405No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA3724230.4786842435760563No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTT3625010.4659312582213344No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGC3617070.4649107109151815No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCC3612190.464283472772357No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGC3482900.44766551795969817No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3262610.41935111417223886No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG3182260.4090235353247091No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3180600.4088101715302237No Hit
GGGGGGGGGATGCGTGCATTTATCAGATC3036730.39031821423347046No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGC3032140.38972825048847776No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG3008640.3867077389400403No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2960050.3804623493171221No Hit
AAATGGATTTTTGGAAGTAGGAGTTGGA2942170.3781641898921833No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC2884580.3707620086124167No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCG2877140.36980572750942203No Hit
GGGGGGGGGGATGCGTGCATTTATCAGA2826780.36333283552732365No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGC2818150.3622236008608124No Hit
TCGGGGGGGGATGCGTGCATTTATCAGA2733400.35133047942549No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC2613130.33587188691780584No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2399410.308401941039846No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2387310.3068466989234164No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2373800.3051102261140806No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGG2318590.2980139519613473No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC2314180.2974471240494916No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2298810.2954715809644072No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCG2286670.293911197551725No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG2272600.292102746594852No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT2193470.28193197728302827No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2123020.2728768692580316No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGT2120320.2725298317609771No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCG1928410.2478631776647798No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1891070.2430637776129221No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGG1822780.23428629958556912No Hit
GGGGGGGGATGCGTGCATTTATCAGAT1774600.2280936082492407No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC1762120.2264895238184109No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATC1644060.21131498792868625No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG1620870.20833432142620692No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCC1617550.20790759383723617No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1569620.20174703560248683No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG1562760.20086530329515573No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1532210.19693863828218702No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG1517900.19509933954779807No Hit
TGGGGGGGGATGCGTGCATTTATCAGA1498170.19256339517117374No Hit
CGGGGGGGGATGCGTGCATTTATCAGAT1470630.18902361270121765No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1420240.18254686474692977No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGC1396760.17952892384661862No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1391620.1788682672781519No Hit
TTAACGCGAACTCGGCCTACAATAGTGA1360140.1748220671273088No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAT1355210.17418840236416855No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1354890.17414727199414726No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1297800.16680935691753893No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC1266620.1628017164885908No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1244230.1599238759111646No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGT1220190.15683395686331625No Hit
TGGGGGGGGGATGCGTGCATTTATCAGAT1188590.1527723328237152No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCA1178610.15148957940867663No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGG1176500.15121837603134886No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC1127140.14487401645556697No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCC1107440.14234192805113213No Hit
TCGTCGTCGCGCCGTCGGGTCCCGGGGG1097540.1410694572285989No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCC1052710.1353073494534307No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC1030360.1324346501722572No Hit
TGGCGGGCGTGGGGGTGGGGGCCGGGCCGC1015050.13046681903155177No Hit
GGGGGGGGGATGCGTGCATTTATCAGA1014160.1303524251899301No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC1013920.13032157741241412No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGC1009340.12973289899148463No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1000120.12854783020524657No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGG997570.12822007256913956No Hit
TTAACGCGAACTCGGCCTACAATAGT977330.1256185766652938No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGC968540.12448877681377189No Hit
TAAATGGATTTTTGGAAGTAGGAGTTGGA964280.12394122876286362No Hit
TAGGGGGGGGATGCGTGCATTTATCAGAT949480.12203894914937961No Hit
CCAACTTCTTAGAGGGACAAGTGGCGT944830.12144127346000794No Hit
TGGGGGGGGATGCGTGCATTTATCAGATC936570.12037959578383374No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTG922960.11863026973386634No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC915060.11761486372396607No Hit
GGGGGGGGATGCGTGCATTTATCAGATC904130.11621000452292683No Hit
ATTAAATGGATTTTTGGAAGTAGGAGT895240.11506735143077325No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC874250.11236945622219016No Hit
CGGGGGGGGATGCGTGCATTTATCAGA871580.11202627469732514No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC868960.11168951979277594No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG863220.1109517437805193No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC836100.10746594492121614No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG831090.10682199756557051No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGG807980.10385161365559646No Hit
TTTGGTGGTTCAGTGGTAGAATTCTCGC801400.10300587042203398No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT791200.10169483987760579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)