FastQCFastQC Report
Sat 1 Jul 2023
SRR2927428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2927428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65453576
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length25-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCG13854322.1166635723615776No Hit
ATGTACACGGAGTCGACCCGCAACGCGA12999161.9860121928250336No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCG12020201.8364466442597422No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC10750151.6424083536703937No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCC10243031.5649305394712123No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCC7650121.1687856443473768No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCG7326931.1194086630194202No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCG6689931.0220877771445214No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC5913930.903530465623452No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAT5290750.8083209999099209No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGC4243150.648268629356477No Hit
TCGGGGGGGGATGCGTGCATTTATCAGAT4174550.6377879185699495No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGC3896040.5952371494568914No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT3863040.5901954081164336No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT3729930.569858856909514No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGC3691600.5640027979525518No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3627560.5542187641512513No Hit
GGGGGGGGGATGCGTGCATTTATCAGAT3488100.5329120596863951No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC3481920.531967879035364No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3378990.516242229454354No Hit
AAATGGATTTTTGGAAGTAGGAGTTGGA3332580.5091517077691828No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT3324570.5079279396438171No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3160110.4828017341634626No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3108120.47485869985163226No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3088610.4718779612591373No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC3069240.4689186118723292No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGT3053540.4665199652345962No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCC3015510.4607097402898201No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTT2998830.45816136921227957No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2846600.43490366362870686No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC2817650.43048068145275975No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCC2739780.4185836996896854No Hit
TGGGGGGGGATGCGTGCATTTATCAGAT2672190.408257296744184No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCC2647950.4045539085595568No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG2619640.4002287056096064No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC2619560.40021648320635683No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2437430.3723906544082481No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2400260.3667118202984051No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGG2333250.3564740297764632No Hit
TCGGGGGGGGATGCGTGCATTTATCAGA2153120.32895376105959434No Hit
GGGGGGGGGATGCGTGCATTTATCAGATC2110930.3225079711458393No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC2086710.3188076385620245No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2070150.3162776010893584No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG2013480.30761955618742665No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2011130.3072605230919698No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGG1969310.3008712617932441No Hit
CGGGGGGGGATGCGTGCATTTATCAGAT1951270.2981151098604605No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1898420.29004068471369693No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1777240.2715267993913732No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC1624290.24815909217855417No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGC1572510.24024814167525393No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT1465810.22394651134110688No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC1432900.21891852020430477No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1427300.21806295197683315No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1421800.21722266175342353No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1419810.21691862947258986No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1419230.2168300170490303No Hit
TAAATGGATTTTTGGAAGTAGGAGTTGGA1414080.2160431998398376No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCC1390730.21247578589136212No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1383730.2114063256070226No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1378780.2106500644059539No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATC1302970.19906780952655664No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1254400.19164728295364641No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGC1167460.17836458622214926No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1166880.1782759737985897No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1145410.1749957863264797No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1127840.17231144101278745No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT1127590.17227324600263247No Hit
GGGGGGGGGGATGCGTGCATTTATCAGA1119880.17109531188945276No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1113440.17011140842786038No Hit
TGGGGGGGGGATGCGTGCATTTATCAGAT1110130.1696057064934084No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCG1103560.1686019416265354No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1063760.162521296009862No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1027100.1569203797207352No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGT998420.15253864815575546No Hit
CGGGGGGGGATGCGTGCATTTATCAGA991890.15154099449050729No Hit
TGGCGGGCGTGGGGGTGGGGGCCGGGCCGC925420.1413857051905002No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC917570.14018638187163374No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG915480.13986707158673806No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC889330.13587187352452676No Hit
CCCCGCCCCTTGCCTCTCGGCGCCC878880.13427532210004844No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGG878380.1341989320797385No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT871400.13313252739621131No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCG856560.1308652715934115No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTG852860.13029998544311772No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCC843500.12886996426291514No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG830910.12694646355151015No Hit
TTAACGCGAACTCGGCCTACAATAGT828920.12664243127067648No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAT826800.1263185375845622No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG818860.12510546406203993No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCG808800.12356849685340339No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG797970.12191388901348951No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC783400.11968788382165706No Hit
TCGTCGTCGCGCCGTCGGGTCCCGGGGG780320.11921732129654766No Hit
ATTAAATGGATTTTTGGAAGTAGGAGT778710.11897134543114955No Hit
TGGGGGGGGATGCGTGCATTTATCAGATC772770.11806383198986715No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGG772170.11797216396549517No Hit
ATGTACACGGAGTCGACCCGCAACGC767440.1172495143733629No Hit
GGGGGGGGATGCGTGCATTTATCAGAT764120.11674228463850471No Hit
ATGCGTGCATTTATCAGATCAAAACCAAC751610.11483100633034932No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCA726190.11094733769779057No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG719050.10985648820776422No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC716770.10950814971515078No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAT707350.108068961732511No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCC705360.10776492945167732No Hit
TATTAAATGGATTTTTGGAAGTAGGAGT700920.10708658607132482No Hit
CCTCGCTTCTGGCGCCAAGCGTCCGTCCCG700050.10695366743598547No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCC699860.10692463922826768No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT697550.10657171733443563No Hit
TTGGGGGGGATGCGTGCATTTATCAGAT682210.10422807151132583No Hit
ATGCGTGCATTTATCAGATCAAAACCAA680650.10398973464795873No Hit
TCGGGGGGGGATGCGTGCATTTATCAGATC680440.10395765083942854No Hit
GGGGGGGGATGCGTGCATTTATCAGATC676090.10329305766273182No Hit
TTGGGGGGGGATGCGTGCATTTATCAGAT668710.1021655409629567No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG663890.10142914116716861No Hit

[OK]Adapter Content

Adapter graph