FastQCFastQC Report
Sat 1 Jul 2023
SRR2927429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2927429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27665962
Total Bases832.5 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA5690442.056837929582929No Hit
ATGTACACGGAGTCGACCCGCAACGCG4941131.7859960915149093No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT4339651.5685881445221386No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT4012191.4502260937103868No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCG3419201.2358868995771772No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAT3007621.0871192550615085No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT2805141.0139318488184146No Hit
GGGGGGGGGATGCGTGCATTTATCAGAT2694510.973944083346894No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCC2454430.8871659695043317No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC2406980.87001493025979No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2328020.8414744443009067No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCC2103420.7602916536934446No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1987230.7182941984811517No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCC1934060.6990756366975419No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCC1932860.6986418907103249No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1917460.6930754838743725No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCG1767710.6389475992195753No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC1751080.6329366027467254No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGC1726900.6241966211043014No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1593530.5759893691750173No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1591240.5751616372494114No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1582430.5719772187932594No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC1542470.5575334774189309No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1532730.5540129058226857No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCG1513900.547206708373271No Hit
TGGGGGGGGATGCGTGCATTTATCAGAT1479840.5348955514360932No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1418880.5128612552854659No Hit
GGGGGGGGGGATGCGTGCATTTATCAGA1414810.5113901334788213No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCC1367930.4944451235782078No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG1345910.48648588471277454No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGC1304830.4716373137503767No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1254760.4535392624337444No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1186710.4289422504086429No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1137840.4112779450792277No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1085430.3923340890875221No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC1081020.3907400725844994No Hit
TCGGGGGGGGATGCGTGCATTTATCAGAT1052500.38043137628830687No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1051080.37991811020343336No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTT1009660.3649466445446574No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGC1000810.36174776788893154No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC998590.36094533781258No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCG976190.3528487460511946No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG963080.3481100711408481No Hit
GGGGGGGGGATGCGTGCATTTATCAGATC957040.34592688300518887No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCG930130.336200129241846No Hit
TCGGGGGGGGATGCGTGCATTTATCAGA919170.33223858255859673No Hit
AAATGGATTTTTGGAAGTAGGAGTTGGA893490.3229564184321514No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC879130.31776592478512045No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC868790.3140284801952667No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG867940.31372124345432123No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC858380.31026573375615857No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGC830360.30013776495463995No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGG814780.2945062962206049No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGG790360.2856795653807375No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG773360.27953483056182904No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGT765320.2766287324474746No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC752150.2718683702377672No Hit
GGGGGGGGATGCGTGCATTTATCAGAT715710.25869695042594215No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG700190.25308716899126804No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT688680.24892682206387762No Hit
TGGGGGGGGATGCGTGCATTTATCAGA676330.24446285294543527No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC671860.24284714914305167No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCC629000.22735518829961524No Hit
GGGGGGGGGATGCGTGCATTTATCAGA623550.22538525860767103No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCG607890.21972487347448827No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG601150.21728866684628567No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGC566390.2047244914165645No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCC562720.203397951605659No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAT541320.1956628148336212No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC539960.19517123604810852No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT535220.19345793939860106No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG531300.19204103584035864No Hit
TGGGGGGGGGATGCGTGCATTTATCAGAT484590.1751574732879341No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC465050.16809464279608277No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG464650.16795006080034375No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC437380.1580931832408358No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGC432550.15634735564228708No Hit
TGGCGGGCGTGGGGGTGGGGGCCGGGCCGC425770.15389669081451063No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGC416770.1506435959103826No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATC411900.14888331011225997No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC410790.14848209507408416No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG401590.14515670917208665No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCG390280.1410686532425657No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCC384710.1390553489518998No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG381790.13799990038300494No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAT381770.137992671283218No Hit
CGGGGGGGGATGCGTGCATTTATCAGAT378950.13697336821325787No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGG373820.13511910411790487No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG368860.13332628737074098No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGG356410.12882617275336386No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC352200.12730444724821063No Hit
TCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTC351490.12704781420577385No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG350830.12680925391280448No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCA340040.1229091545777443No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC339850.12284047812976828No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG337000.12181033141012772No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG335150.12114163967983473No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGT334950.12106934868196521No Hit
CGGGGGGGGATGCGTGCATTTATCAGA326350.11796083577357622No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT326210.11791023207506757No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG319360.11543426539803678No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC319150.11535835985027378No Hit
GGGGGGGGATGCGTGCATTTATCAGATC314330.11361614680161854No Hit
TAGGGGGGGGATGCGTGCATTTATCAGAT312090.11280648762548001No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC311560.11261491648112579No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG311490.11258961463187146No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG311190.1124811781350672No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCC307380.11110403462565299No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTG307130.11101367087831611No Hit
TTGCGTGCATTTATCAGATCAAAACCAAC298120.10775696142429458No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGC294980.10662199275774326No Hit
TAAATGGATTTTTGGAAGTAGGAGTTGGA291920.10551594049033972No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT283870.10260622782609186No Hit
CCAACTTCTTAGAGGGACAAGTGGCGT276780.10004351195161765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)