FastQCFastQC Report
Sun 2 Jul 2023
SRR2954350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2954350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12753389
Total Bases650.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT176166813.813332283677696No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT5572134.369136705545483No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA5005063.9244941089776213No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT3864773.0303866682024676No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3514972.756106631735298Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG3181332.4944977370328782No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT3121102.44727107437874No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG2600182.038814937739294No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA2045571.6039422932994516No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1365891.0710015980850267No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA966760.7580416468124669No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA866450.679388043444766No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA857050.6720174535568546No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT670700.525899429555548No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT629990.49397850249843395No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA616690.4835499019123466No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATTCG528270.41421931064754625No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT487520.38226701937814334No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT470590.36899211652683067No Hit
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT457160.35846158225080405No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCG449110.3521495345276459No Hit
GCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT441660.3463079499888226No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA416440.32653281414061786No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG403820.31663740516344324No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG400090.31371269236749544No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG344140.26984200042827833No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGTA305940.23988917769229812No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG295440.23165607196643967No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC290180.2275316780504382No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA255650.20045652179197232Illumina Single End Adapter 1 (95% over 22bp)
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT233120.18279062922020178No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG224020.1756552709244578No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT192840.15120686744519438No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG191830.1504149210848975No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA186160.14596904399293395No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT179880.1410448626635634No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATTCGT179590.14081747212446827No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT177570.13923357940387454No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT176040.13803389828382087No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG174640.13693615085370642No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATTCGTA174130.13653625714702186No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT170350.1335723390857128No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC165030.1294008988512779No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG153630.12046209834891729No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT142120.11143704626276199No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC136630.10713230812609888No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT136140.1067480965255588No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTA135440.10619922281050159No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC132360.10378417846424977No Hit
TAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT130520.10234142469895649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)