FastQCFastQC Report
Sun 2 Jul 2023
SRR2954351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2954351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12812990
Total Bases653.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT161144312.576635117954513No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT4841043.7782281887365867No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA4634313.616884115261153No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT3369232.6295423628676833No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3039702.372358052257904Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG2805182.189325052154103No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT2723252.125382131727255No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG2280581.7798968078489095No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1885821.4718032246961872No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1233530.962718303846331No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA890580.6950602474519999No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA802620.6264111655437178No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA799130.6236873672733687No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT587530.45854246354676No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT570730.44543076986714264No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA568870.4439791180668993No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCACTTCGTAT500080.3902914151966091No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCATTTCGTAT474320.3701868182211958No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATTCG464410.3624524798661358No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT439310.34286298514242186No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATAAATTCGT424210.3310780699899087No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT423330.330391266987643No Hit
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT396680.3095920624303929No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCG393710.30727410229774627No Hit
GCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT384910.3004060722750896No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA383640.2994148906695471No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG350170.27329296284473803No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG348200.2717554606692115No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG292150.22801079217263107No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATAAATTCGT291710.22766739067149822No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGTA277940.21692048460195476No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG254480.19861094092791767No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC246040.19202387576982421No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATAAATTCGT240230.18748941503895655No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA221310.17272315049024467Illumina Single End Adapter 1 (95% over 22bp)
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT201830.15751982948554552No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG196080.1530321962321051No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAAATCGTATG175160.13670501576915303No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA171420.13378610300952393No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG171220.1336300114180999No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT168360.13139790166073648No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATTCGTA163770.12781559963755532No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT161970.12641077531473918No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT160320.12512301968549105No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT159800.12471718154778862No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATTCGT155010.12097878793318342No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG152620.11911349341566645No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT146450.11429806782023556No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAAATCGTATG143660.11212059011987054No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT139190.10863194305154378Illumina Single End Adapter 1 (95% over 22bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC138790.10831975986869576No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG132780.10362920754640406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)