FastQCFastQC Report
Sun 2 Jul 2023
SRR2954352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2954352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14366124
Total Bases732.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8815146.1360600813413555Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT7632265.312678632037423No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT3582572.4937624094014503No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT2877802.0031847142625248No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1871751.3028914410038503No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1721941.1986113999851318No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1596941.1116011528231275No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATTCG956340.6656910381672886No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA824220.5737246873269366No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA801370.5578192141457222No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA771350.5369228331872953No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA713230.4964665486668499No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA619060.43091650886488236No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA600950.4183104642560513Illumina Single End Adapter 1 (95% over 22bp)
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCG556560.3874113852838803No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG461850.32148546121417304No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG456140.3175108331238127No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT375030.2610516239453314No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA372410.2592278891648158No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA364950.2540351176141874No Hit
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT341340.23760062213022803No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT339960.2366400290015595Illumina Single End Adapter 1 (95% over 22bp)
GCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT338290.23547757209947512No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGGACTGTAGGCACCATCAA320010.22275319355450365No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT318160.22146544189650597No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG273720.19053155882547024No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG264050.1838004461050176No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA256140.17829443766460598No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC235110.1636558336820704No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC195620.13616755639865005No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGTA184280.12827398677611304No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA183710.12787722004905427No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC183270.12757094397904403Illumina Single End Adapter 1 (95% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATT171670.11949639304241004No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA170360.11858452565215225No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA168100.1170113803834632No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT153610.10692515253244367No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA150530.1047812200423719Illumina Single End Adapter 1 (95% over 22bp)
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT149650.10416866790235138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)