FastQCFastQC Report
Sun 2 Jul 2023
SRR2954353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2954353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14252998
Total Bases726.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8819856.188066538702945Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT7492075.2564870913473785No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT3500392.455897348754276No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT2832551.9873362783043962No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1829891.2838632265296044No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1699721.1925350722704093No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1581401.109520958327504No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATTCG930400.6527749460148665No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA804930.5647443436110775No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA779980.5472392545063152No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA754020.5290255425560293No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA701500.49217715458881006No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA602730.42287945315083886No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA599190.42039576515761806Illumina Single End Adapter 1 (95% over 22bp)
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCG552650.3877429857213198No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG453450.3181435933689179No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG446660.31337968334802263No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT370690.26007861644266No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA361010.2532870628340788No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA353040.24769525681544333No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT338280.23733954077591254Illumina Single End Adapter 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT333730.2341472299371683No Hit
GCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT332470.23326320539720835No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT311200.21834002923455123No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGGACTGTAGGCACCATCAA311130.218290916760109No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG268230.18819198599480613No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG261420.18341404383835597No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA255310.179127226426328No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC230790.16192382823599638No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC191460.1343296336672467No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA182640.1281414618875271No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGTA182260.12787485131198362No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC177140.12428262460992417Illumina Single End Adapter 1 (95% over 22bp)
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA170970.11995371079123143No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATT168650.11832598306686073No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA166110.11654390185138594No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA161180.1130849804370982Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT152100.10671439089516466No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT148210.10398514052973276No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA147000.10313619632866013Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA146600.10285555361756171Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)