FastQCFastQC Report
Sun 2 Jul 2023
SRR2954355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2954355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301647
Total Bases729.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7663555.3585087088221375Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT6823034.770800174273634No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT3100492.1679251347764352No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT2470371.7273325233100774No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1605971.1229266111798173No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1501841.0501168152171565No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1451391.0148411578051115No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATTCG833450.5827650479696499No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA750170.5245339924835231No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA696540.4870348149412442No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA688290.48126624856563727No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA647250.452570252922618No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA559200.39100391724114014No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA531400.37156559660576155Illumina Single End Adapter 1 (95% over 22bp)
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCG480900.33625497818537964No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG402590.28149904692795175No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG392640.27454180626888636No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT349310.24424459644403193Illumina Single End Adapter 1 (95% over 22bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA337960.23630844755153027No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA332020.23215507976109326No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT324710.2270437803422221No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGGACTGTAGGCACCATCAA291190.20360592035308941No Hit
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT290560.20316541164804308No Hit
GCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT289130.2021655268096045No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT272820.19076124589007126No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATAAATTCGT270920.18943272757326482No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAACAAAAAAA243820.1704838610546044Illumina Single End Adapter 1 (95% over 22bp)
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG232570.16261763417877675No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA231470.1618484919953625No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG228400.15970188608346997No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC216040.15105952482256066Illumina Single End Adapter 1 (95% over 22bp)
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC215790.1508847197808756No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATAAATTCGT211920.14817873773559087No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCACTTCGTAT209760.14666842217543197No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCATTTCGTAT197420.13804004531785743No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATTC173910.1216013791977945No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGTA168880.1180843017590911No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA168410.11775566828072319No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA157480.1101131918582524No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA154130.10777080429967262No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATT145560.10177848747070879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)