FastQCFastQC Report
Sun 2 Jul 2023
SRR2954801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2954801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498569
Total Bases108.9 Mbp
Sequences flagged as poor quality0
Sequence length24-48
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCAAAAGGGACAAGCATCAAGCACGCAGC1033662.954522263245344No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAGC468451.3389760213390103No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGC259890.7428465752712037No Hit
GCCTGGTGATAGCTGGTTGTCCAAGATAGAAT249200.712291225355281No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCGTGC204510.5845532845000342No Hit
CGCCCGATCTCGTCTGATCTCGGAAGCTAAGC153440.43857931628617297No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGC153110.4376360734917619No Hit
ACATCACCTCTAGCATCACCAGTATTAGAGGC97240.2779422100864668No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCGTG82810.23669677516721838No Hit
GCCTGGTGATAGCTGGTTGTCCAAGATAGAA82740.2364966933623433No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC82360.23541053499302142No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT79890.22835050559242936No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACAC70000.2000818048750789No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTA68390.1954799233629521No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCC67130.1918784508752007No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC59920.17127002497306756No Hit
GGGTCGGGCCTGGTTAGTACTTGGATGGGAGAC51710.14780328757271902No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTT50300.1437730683602353No Hit
GCCTGGTGATAGCTGGTTGTCCAAGATAGAATC50230.14357298655536022No Hit
AGCTTGGGCTGCAGGTCGACCCGTGC47310.1352267169805712No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT46540.13302581712694533No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGT43500.12433655017237047No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCC43120.12325039180304861No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG42690.12202131785881598No Hit
TATACCCACACCCACCCAAGAACAGGGTTTAAAA42400.12119240752433352No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGC41850.11962033620031504No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG41660.11907725701565412No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTA41240.11787676618640365No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTAAAA40310.11521853649306331No Hit
GATCCAAAGGGACAAGCATCAAGCACGCAGC39530.11298905352445528No Hit
GGTACACGGAGTCAAGACCCGCAACGCGG37210.10635777084859552No Hit
TTTGTGACTCTAGATCACCTCGGGCCGATCGCAC36460.10421403722493397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)