FastQCFastQC Report
Sun 2 Jul 2023
SRR3029399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3029399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24208026
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAA438078318.096407365061488No Hit
ACAAGCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGC315164413.019004523541078No Hit
ACAAGCCAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAG2462111.0170635143898143No Hit
ACAAGCCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCC2228230.9204509281343303No Hit
ACAAGCCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCC1849990.7642052268119672No Hit
ACAAGCCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA1546990.6390401266092494Illumina Paired End PCR Primer 2 (100% over 22bp)
ACAAGCCTTACCTATACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGC1518160.6271308532137234No Hit
ACAAGCCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCA1163770.4807372563132574No Hit
ACAAGCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTG662100.27350433281920633No Hit
ACAAGCCAAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCA648530.2678987539091374No Hit
ACAAGCCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTG637720.2634332927434893No Hit
ACAAGCCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTG582270.24052766632025263No Hit
ACAAGCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGGGCCAA471110.19460901107756576No Hit
ACAAGCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAG378650.15641506663946908No Hit
ACAAGCCTAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCA358150.14794680078416966No Hit
ACAAGCCATGATTAATAGGGACGGTCGGGGGCATGGAATTCTCGGGTGCC348050.14377463077741243No Hit
ACAAGCCAGAATCCATGCTAGAACGCGGTGATTTTGGAATTCTCGGGTGC342980.14168028405124813No Hit
ACAAGCCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTG333040.13757420782677612No Hit
ACAAGCCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGC329570.13614079892346448No Hit
ACAAGCCCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGC306300.12652828446235145No Hit
ACAAGCCTCGGGATAAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGT281590.11632092596067106No Hit
ACAAGCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCC276490.11421418665032829No Hit
ACAAGCCTTACCTATACTCTACCGTCAGGGTTGGAATTCTCGGGTGCCAA273050.11279317033119513No Hit
ACAAGCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGCGCCAA267150.11035596210942603No Hit
ACAAGCCGACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGTG266690.11016594248535588No Hit
ACAAGCCTCGAAGAGTCGAGTTGTTTGGGAATGCATGGAATTCTCGGGTG264240.1091538814441128No Hit
ACAAGCCTCGAAGAGTCGAGTTGTTTGGGAATGCTGGAATTCTCGGGTGC260770.10772047254080114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)