FastQCFastQC Report
Sun 2 Jul 2023
SRR3029401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3029401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24737947
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCACCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAA261263110.561228059870935No Hit
ATTCACCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGC17760397.17941145237315No Hit
ATTCACCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCC8840723.5737484602097336No Hit
ATTCACCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCC8623743.4860370587745217No Hit
ATTCACCAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAG8416163.4021254876162517No Hit
ATTCACCTTACCTATACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGC2900261.1723931658516369No Hit
ATTCACCATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCC1047290.42335364369565515No Hit
ATTCACCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA955770.3863578493397209Illumina Paired End PCR Primer 2 (100% over 22bp)
ATTCACCTACCTATACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGCC543790.2198201815211262No Hit
ATTCACCATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCC483860.19559424231929998No Hit
ATTCACCTTACCTATACTCTACCGTCAGGGTTGGAATTCTCGGGTGCCAA459760.1858521242688409No Hit
ATTCACCATGAGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGGTGCC442310.17879818402068692No Hit
ATTCACCACCTATACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGCCA432060.17465475206976552No Hit
ATTCACCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTG417990.16896713377225683No Hit
ATTCACCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGC402010.1625074223014545No Hit
ATTCACCAAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCA395570.1599041343244854No Hit
ATTCACCATGAAAGTGAACGTAGGTTGGGGCCTCTGGAATTCTCGGGTGC387580.1566742785890842No Hit
ATTCACCATGGATTTGAGTAAGAGCATAGCTGTTTGGAATTCTCGGGTGC375800.15191236362500088No Hit
ATTCACAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAG374560.15141110941825528No Hit
ATTCACCAATGACCCACTCGGCACCTTACGAGAAATTGGAATTCTCGGGT366080.1479831774237369No Hit
ATTCACCATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTG365990.14794679607002148No Hit
ATTCACCACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGTGC346970.1402582033181654No Hit
ATTCACCATGATGCCCTGACGAGTAGGCAGGCGTTGGAATTCTCGGGTGC341750.1381480848026718No Hit
ATTCACCTAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAA335590.13565798325948392No Hit
ATTCACCATGGATTTGAGTAAGAGCATAGCTGTTGGAATTCTCGGGTGCC331080.1338348732010785No Hit
ATTCACCAATGACCCACTCGGCACCTTACGAGAAATCTGGAATTCTCGGG328790.13290916986765314No Hit
ATTCACCTTTACCTATACTCTACCGTCAGGGTTGATGGAATTCTCGGGTG322080.13019673782953775No Hit
ATTCACCAATGACCCACTCGGCACCTTACGAGAATGGAATTCTCGGGTGC320590.129594424306916No Hit
ATTCACCTCGAAGAGTCGAGTTGTTTGGGAATGCATGGAATTCTCGGGTG312430.12629584823671908No Hit
ATTCACCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTG310390.12547120421916985No Hit
ATTCACAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCC305990.12369256025974992No Hit
ATTCACCAGAATCCATGCTAGAACGCGGTGATTTTGGAATTCTCGGGTGC304630.12314279758138377No Hit
ATTCACCTCAGAATCCATGCTAGAACGCGGTGATTTTGGAATTCTCGGGT297340.12019590793043579No Hit
ATTCACCATGGGGAAGCTCCGTTTCAAAGGCCTGTGGAATTCTCGGGTGC297070.12008676386928957No Hit
ATTCACCTCGAAGAGTCGAGTTGTTTGGGAATGCTGGAATTCTCGGGTGC290520.11743900979333491No Hit
ATTCACCAATGACCCACTCGGCACCTTACGAGAAATGGAATTCTCGGGTG275290.11128247627016098No Hit
ATTCACCTCGGGATAAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGT263950.10669842570201965No Hit
ATTCACCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGGGCCAA263700.10659736638614352No Hit
ATTCACCAAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGG261990.10590612066555079No Hit
ATTCACCATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTG257970.10428108686626258No Hit
ATTCACCATGGATTTGAGTAAGAGCATAGCTGTTGGTGGAATTCTCGGGT255760.10338772251391759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)