FastQCFastQC Report
Sun 2 Jul 2023
SRR3029402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3029402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26409935
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTCCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGT417860515.822094980544254No Hit
TGACTCCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG16962226.4226663185653425No Hit
TGACTCCTTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGG11563444.37844318814113No Hit
TGACTCCTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGG9731643.6848405723073534No Hit
TGACTCCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTG9372373.5488046449186643No Hit
TGACTCCTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGC5739522.173242758832992No Hit
TGACTCCTTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGT4551001.7232151461183072No Hit
TGACTCCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGT4438811.6807349204002207No Hit
TGACTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAG3863001.4627071213920064No Hit
TGACTCCTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGC3421971.2957131473439825No Hit
TGACTCCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGC3381451.2803704363528348No Hit
TGACTCCTTTTTCCACGTTCTAGCATTCAAGGTCCCATGGACTTCTCGGG3293451.2470496424924937No Hit
TGACTCCTCCAACGGGGCCTTTCCTTCTGGCTAACCTGGAATTCTCGGGT3041481.1516423648903338No Hit
TGACTCCTTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTG3011741.1403814511470778No Hit
TGACTCCTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGACTTCTCGGG2785711.054796234826023No Hit
TGACTCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGC2426360.9187300158065517No Hit
TGACTCCTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCC2074110.7853521790189941No Hit
TGACTCCGACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGTG1639370.620739884441215No Hit
TGACTCCTTTTCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGGG1443260.546483738032676No Hit
TGACTCCTGATGTATTTATTAGATAAAAAATCAATGTGGAATTCTCGGGT1350550.5113795244100373No Hit
TGACTCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAA1301660.4928675515483094No Hit
TGACTCCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA1286250.48703262616890197Illumina Paired End PCR Primer 2 (100% over 22bp)
TGACTCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTG1241650.4701450420078656No Hit
TGACTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGC1093340.41398814499164804No Hit
TGACTCCTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCC1081440.40948226491280654No Hit
TGACTCCTGACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGT1076610.4076534077043355No Hit
TGACTCCTCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAA934710.35392362760453594No Hit
TGACTCCAGAGATGTATTTATTAGATAAAAAATCATGGAATTCTCGGGTG888470.336415065012466No Hit
TGACTCCCCAACGGGGCCTTTCCTTCTGGCTAACCTGGAATTCTCGGGTG792480.3000688945277601No Hit
TGACTCCTTTCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGGGT787600.2982211050500503No Hit
TGACTCCTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCC740180.2802657409039439No Hit
TGACTCCACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGTGC651610.24672911917428042No Hit
TGACTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG634510.24025428309460056Illumina Small RNA Adapter 2 (100% over 21bp)
TGACTCCATGTATTTATTAGATAAAAAATCAATGTGGAATTCTCGGGTGC633410.2398377731713463No Hit
TGACTCCTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGC616480.23342730680707846No Hit
TGACTCCTTTTCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCC579850.2195575263627116No Hit
TGACTCCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCA544250.20607775066466463No Hit
TGACTCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAA512860.19419207203652716No Hit
TGACTCCCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGT504490.19102281016594702No Hit
TGACTCCTTTTTCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGC495360.18756577780293665No Hit
TGACTCCGATGTATTTATTAGATAAAAAATCAATGTGGAATTCTCGGGTG489250.18525225450195165No Hit
TGACTCCAGAGATGTATTTATTAGATAAAAAATCTGGAATTCTCGGGTGC469110.1776263364525509No Hit
TGACTCCTCCAACGGGGCCTTTCCTTCTGGCTTGGAATTCTCGGGTGCCA462930.17528630797463152No Hit
TGACTCCTCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGT435150.16476753918553755No Hit
TGACTCCTTTTTCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGG428830.16237450035374945No Hit
TGACTCCTCCGTGATAGTTTAATGGTCAGAATGGGCTGGAATTCTCGGGT407850.15443052018113637No Hit
TGACTCCTTTTCCACGTTCTAGCATTCAAGGTCCCATTGGACTTCTCGGG403960.1529575896343554No Hit
TGACTCCTTTGGAAGAGATGTATTTATTAGATAATGGAATTCTCGGGTGC379830.14382087649969605No Hit
TGACTCCTACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCC378310.1432453355148356No Hit
TGACTCCTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGG374050.1416323061756873No Hit
TGACTCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG321360.12168148085180822No Hit
TGACTCCTTTGGAAGAGATGTATTTATTAGATAAATGGAATTCTCGGGTG320960.12153002269789759No Hit
TGACTCCTAATGACCCACTCGGCACCTTACGAGATGGAATTCTCGGGTGC315510.11946640535086513No Hit
TGACTCCCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGC308760.11691054900362308No Hit
TGACTCCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTG300010.11359740188682782No Hit
TGACTCCATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCA280710.10628954596063943No Hit
TGACTCCGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCC280530.10622138979137966No Hit
TGACTCCTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCA272670.10324523706703556No Hit
TGACTCCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCA270810.10254095665135109No Hit
TGACTCCTATCTGGTTGATCCTGCCAGTAGTCATTGGAATTCTCGGGTGC267550.10130657269697936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)