FastQCFastQC Report
Sun 2 Jul 2023
SRR3029403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3029403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24955817
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGT289398611.596438617898183No Hit
TCTACCCTTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGG12960585.193410418100116No Hit
TCTACCCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG9234433.700311634758341No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGG9117243.6533526431933687No Hit
TCTACCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAG7065682.8312757702943565No Hit
TCTACCCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTG6709142.6884072759469264No Hit
TCTACCCTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGC4213501.688383914660057No Hit
TCTACCCTTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGT3721441.491211447815954No Hit
TCTACCCTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGC3274471.3121069127891105No Hit
TCTACCCTTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTG3214271.2879842803783983No Hit
TCTACCCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGT2792931.1191498959941883No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTAACCTGGAATTCTCGGGT2316340.9281763846881872No Hit
TCTACCCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGC1985790.7957222959280394No Hit
TCTACCCTTTTTCCACGTTCTAGCATTCAAGGTCCCATGGACTTCTCGGG1970040.7894111420996556No Hit
TCTACCCTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCC1926840.7721005487418023No Hit
TCTACCCGACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGTG1829280.7330074587419839No Hit
TCTACCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGC1666850.667920429132815No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAA1512830.6062033553139135No Hit
TCTACCCTTTTCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGGG1405400.5631552755816409No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGACTTCTCGGG1389490.5567800084445242No Hit
TCTACCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG1344670.5388202678357515Illumina Small RNA Adapter 2 (100% over 21bp)
TCTACCCTGACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGT1264600.5067355638967861No Hit
TCTACCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGC1177160.4716976406743166No Hit
TCTACCCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA1152400.4617761061479173Illumina Paired End PCR Primer 2 (100% over 22bp)
TCTACCCTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCC1150200.4608945481528415No Hit
TCTACCCTTTTTCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGC1041460.41732154070531935No Hit
TCTACCCTGATGTATTTATTAGATAAAAAATCAATGTGGAATTCTCGGGT984310.39442106824232603No Hit
TCTACCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAA884930.35459868935567207No Hit
TCTACCCAGAGATGTATTTATTAGATAAAAAATCATGGAATTCTCGGGTG820200.32866084889146285No Hit
TCTACCCTTTTCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCC792120.31740896320885825No Hit
TCTACCCATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCA778250.31185114075808457No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTTGGAATTCTCGGGTGCCA776140.3110056464991709No Hit
TCTACCCTTTGGAAGAGATGTATTTATTAGATAATGGAATTCTCGGGTGC750020.300539148848543No Hit
TCTACCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTG617150.24729705302775704No Hit
TCTACCCTTCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCA581760.23311599055242307No Hit
TCTACCCTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCC576760.23111244965452346No Hit
TCTACCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAA553760.22189616152418495No Hit
TCTACCCTTTTGCTGGCTCCGGTGCGCTTGTTGGAATTCTCGGGTGCCAA551690.22106669559245445No Hit
TCTACCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGA537230.2152724553157286RNA PCR Primer, Index 1 (100% over 22bp)
TCTACCCTTTTTCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGG532110.21322082943627932No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTATGGAATTCTCGGGTGCC527500.21137356472841584No Hit
TCTACCCTTTCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGGGT501080.20078685462391394No Hit
TCTACCCAGAGATGTATTTATTAGATAAAAAATCTGGAATTCTCGGGTGC484370.1940910209431332No Hit
TCTACCCACGGCCCGTGAAAATCCACAGGAAGGATGGAATTCTCGGGTGC459070.18395310399976086No Hit
TCTACCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG449400.18007825590322288No Hit
TCTACCCTAATGACCCACTCGGCACCTTACGAGATGGAATTCTCGGGTGC448170.17958538484233955No Hit
TCTACCCGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCC437960.17549415432882842No Hit
TCTACCCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCA435810.17463263174273155No Hit
TCTACCCTTTGGAAGAGATGTATTTATTAGATAAATGGAATTCTCGGGTG433860.1738512507925507No Hit
TCTACCCACGTTCTAGCATTCAAGGTCCCATTGGAATTCTCGGGTGCCAA422270.16920704299121925No Hit
TCTACCCTACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCC405980.1626795067458621No Hit
TCTACCCTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGG398780.15979440785288657No Hit
TCTACCCCCAACGGGGCCTTTCCTTCTGGCTAACCTGGAATTCTCGGGTG393520.1576866828282961No Hit
TCTACCCACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCA390260.15638037416286552No Hit
TCTACCCTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGC389250.1559756589014898No Hit
TCTACCCTTGCTGGCTCCGGTGCGCTTGTTGGAATTCTCGGGTGCCAAGG385310.15439686867394484Illumina Small RNA Adapter 2 (100% over 21bp)
TCTACCCTAATGACCCACTCGGCACCTTACGAGTGGAATTCTCGGGTGCC370630.1485144725977114No Hit
TCTACCCAACGGGGCCTTTCCTTCTGGCTAACCTGGAATTCTCGGGTGCC362540.14527274342490973No Hit
TCTACCCCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGT341970.13703017617095045No Hit
TCTACCCTTTCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCC332350.13317536348339146No Hit
TCTACCCTCCGTGATAGTTTAATGGTCAGAATGGGCTGGAATTCTCGGGT329840.13216958595264583No Hit
TCTACCCTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCA328730.1317247998733121No Hit
TCTACCCAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGC322870.1293766499409737No Hit
TCTACCCTCCAACGGGGCCTTTCCTTCTGGCTAATGGAATTCTCGGGTGC305150.12227610099881724No Hit
TCTACCCGACGGCCCGTGAAAATCCACAGGAAGGAATGGAATTCTCGGGT303620.12166301748405993No Hit
TCTACCCAGTTGAACTTTGGGCCCGGTTGGCTGGAATTCTCGGGTGCCAA302170.12108199062366902No Hit
TCTACCCATGTATTTATTAGATAAAAAATCAATGTGGAATTCTCGGGTGC290330.1163376057774426No Hit
TCTACCCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCA285090.11423789491644372No Hit
TCTACCCGATGTATTTATTAGATAAAAAATCAATGTGGAATTCTCGGGTG270570.10841961214894306No Hit
TCTACCCTATCTGGTTGATCCTGCCAGTAGTCATTGGAATTCTCGGGTGC264770.10609550470737944No Hit
TCTACCCTCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGT264550.10600734890787186No Hit
TCTACCCTAATGACCCACTCGGCACCTTACGAGAATGGAATTCTCGGGTG253220.1014673252332312No Hit
TCTACCCTTCCAACGGGGCCTTTCCTTCTGGCTTGGAATTCTCGGGTGCC251650.10083821339129069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)