FastQCFastQC Report
Sun 2 Jul 2023
SRR3031124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65562560
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG15538312.3699974497640115No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA3937630.6005912520804557No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA2102680.32071352918494944No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2001220.305238233528404No Hit
TGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAG1590820.2426415319963101No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC1570280.23950864639818822No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1528210.23309187438684517No Hit
TGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGGAATTCTCGGGTGCCA1376440.20994299185388732No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1035650.15796363046226383No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC923650.14088070996617583No Hit
TGGAACGTACCTTGAGCATATATGCTGTGTGGAATTCTCGGGTGCCAAGG875160.13348472054782484Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTGGAATTCTCGGGTGC844050.12873963432788468No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA744240.11351600669650484RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGTGGAATTCTCGGGTGCCAAG743240.11336348062064691No Hit
TATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCA728740.11115185252070695No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG728190.11106796317898507Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG709790.10826148338319919No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAGG709090.10815471513009865Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)