FastQCFastQC Report
Tue 4 Jul 2023
SRR3031125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55488992
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG13800402.48705184624727No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC2993140.5394114926434418RNA PCR Primer, Index 7 (100% over 50bp)
GGCTAACTTGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1901540.3426877893186454RNA PCR Primer, Index 7 (100% over 35bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1871890.33734438715340154No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1332840.2401989929822477No Hit
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC1318000.23752458866075638RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1109470.19994416189791303No Hit
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA877290.1581016285175986RNA PCR Primer, Index 1 (100% over 28bp)
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC815810.14702195347142008No Hit
GAATACAAGCTTGGGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC803460.1447962868022544RNA PCR Primer, Index 1 (100% over 31bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC794830.1432410233727079RNA PCR Primer, Index 1 (100% over 26bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG738670.13312009704555455Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAAGG719260.12962210594850956Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA643750.11601400147978899RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)