FastQCFastQC Report
Sun 2 Jul 2023
SRR3031126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66626458
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG26879374.034338730718658No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA7792041.1695113673910145RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA5991740.8993033968577467No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG3407800.5114784880204798Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCCA2214120.33231843121541893No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC1917890.2878571152619279No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG1578770.2369584167298823No Hit
TCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAAGG1388830.20845022258274634Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAG1156090.17351815400422457No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1114460.1672698854860332No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTG970640.14568386631028774No Hit
TGGAACGTACCTTGAGCATATATGCTGTGTGGAATTCTCGGGTGCCAAGG955980.14348353922701398Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCC938590.14087346501295328No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA918850.13791067806726273No Hit
ACGGCAATTTTTGGAAGCCCGAGAGGGCTAATGGAATTCTCGGGTGCCAA877260.13166841317003525No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG819570.12300969083483321No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA817410.12268549530278197No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA731140.10973718578886484No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG721610.10830682309421283No Hit
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC692900.103997724147365RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)