FastQCFastQC Report
Sun 2 Jul 2023
SRR3031127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64252472
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG20713913.223830827862934No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA3786430.5893049531230486No Hit
TGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAG1495730.2327894870721861No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA1296930.20184904325548753RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1240180.19301669825248124No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA1232380.1918027371771782RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCCA1212150.18865422018315497No Hit
TGGAACGTACCTTGAGCATATATGCTGTGTGGAATTCTCGGGTGCCAAGG1164300.18120703589427659Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAGG1089270.16952966416607287Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA1054640.16413998826379786No Hit
TGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAGGA964780.1501545341321654RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGATAGTATAGTGGTTAGTATCCCTGGAATTCTCGGGTGCCAAGGA898790.13988411216303084RNA PCR Primer, Index 1 (100% over 22bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG799940.12449949007409397Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTAGTTACATATAAAAGCATTATTTATGGAATTCTCGGGTGCCAAGGA710380.11056072675305005RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG696120.10834135688973959No Hit
TCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAAGG692760.1078184198111475Illumina Small RNA Adapter 2 (100% over 21bp)
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC664540.10342637089511514No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)