FastQCFastQC Report
Sun 2 Jul 2023
SRR3031128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40064255
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG38867889.70138593616679No Hit
AGTCACTTAGCGATGTACACTGACTGTGTGGAATTCTCGGGTGCCAAGGA14116333.523422562081836RNA PCR Primer, Index 1 (100% over 22bp)
AATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAG5793911.4461544336716108No Hit
ATTAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAG2614020.6524569095319506No Hit
ATTAATTTGATAAAGTGCTGATAGATTTATGGAATTCTCGGGTGCCAAGG2138700.5338174889312182Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTATCATAAAATATAACTTATAGTTTATTTGGAATTCTCGGGTGCCAA2107930.5261373261527014No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1929890.4816987112327435RNA PCR Primer, Index 1 (100% over 22bp)
TAATTTTTCGGAATTATATAATGGCATATGGAATTCTCGGGTGCCAAGGA1805140.4505612297046332RNA PCR Primer, Index 1 (100% over 22bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA1701660.42473271997694706RNA PCR Primer, Index 1 (100% over 22bp)
ATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAGG1700560.424458161021589Illumina Small RNA Adapter 2 (100% over 21bp)
TTAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGG1446430.36102755436236117Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC1415470.3532999677642827RNA PCR Primer, Index 13 (100% over 50bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGATTGGAATTCTCGGGTGCCAAG1384320.3455249573466423No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG1356840.33866597544369664Illumina Small RNA Adapter 2 (100% over 21bp)
TTAATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAG1282300.32006086223243135No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG1130890.2822690700226424No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCTGGAATTCTCGGGTGCCAAGG1121360.27989039107304003Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCTTACTTACTTGATTAAATGGAACGTGGAATTCTCGGGTGCCAAG1013510.25297113349543127No Hit
ATTTATCATAAAATATAACTTATAGTTTATTCTGGAATTCTCGGGTGCCA1011860.25255929506239416No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA991400.24745249849273374RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA915450.2284954506205095No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG907100.22641129855029127Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGTTGTTTCGCAAAAGTTGACCGAACTTGTGGAATTCTCGGGTGCCAA889480.2220133632835554No Hit
CTGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAAG864430.21576090707290077No Hit
TCCCATATTGTCTAGTGGTTAGGATACCCTGGAATTCTCGGGTGCCAAGG790020.19718824173817784Illumina Small RNA Adapter 2 (100% over 21bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG693390.17306948550522155No Hit
CGTTAATTTTTCGGAATTATATAATGGCATATGGAATTCTCGGGTGCCAA682670.17039378368573183No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCTGGAATTCTCGGGTGCCAAGG680140.16976229808840823Illumina Small RNA Adapter 2 (100% over 21bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA658630.16439342251590602No Hit
ATTAATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAA595520.1486412264498616No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTTGGAATTCTCGGGTGCCAA574860.14348451007013607No Hit
AATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGGA550990.1375265807388656RNA PCR Primer, Index 1 (100% over 22bp)
ATTGTTTCCTTACTTACTTGATTAAATGGAATGGAATTCTCGGGTGCCAA526390.13138644410085748No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG492150.12284017261771123No Hit
TAATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGG489590.12220119904887786Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGCATTTTAACACAGAATAAATGTTATTGGAATTCTCGGGTGCCAA488580.12194910400804906No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG469360.11715181026079231Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA461620.1152199136112727No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA458680.11448609240331563RNA PCR Primer, Index 1 (100% over 22bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATG457710.11424398132449985RNA PCR Primer, Index 13 (100% over 49bp)
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG456490.11393947048310271No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGGAATTCTCGGGTGCCA418750.10451960232381707No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG417600.10423256341594273Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG411120.10261516156983327No Hit
TGTTTCCTTACTTACTTGATTAAATGGAACGTGGAATTCTCGGGTGCCAA408650.10199865191552919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)