FastQCFastQC Report
Sun 2 Jul 2023
SRR3031129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43033445
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG28601526.646346812345608No Hit
ACGGTTGTTTCGCAAAAGTTGACCGAACTTGTGGAATTCTCGGGTGCCAA6300361.464061266765884No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCTGGAATTCTCGGGTGCCAAGGA4747431.1031954332264127RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATTGTCTAGTGGTTAGGATATCCTGGAATTCTCGGGTGCCAAGG4027550.9359115915539645Illumina Small RNA Adapter 2 (100% over 21bp)
CTGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAAG2122930.4933209507163556No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC2065290.4799267174635914RNA PCR Primer, Index 8 (100% over 50bp)
TCCCATATTGTCTAGTGGTTAGGATACCCTGGAATTCTCGGGTGCCAAGG1924970.4473195208982223Illumina Small RNA Adapter 2 (100% over 21bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1870180.43458756323134257No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCTGGAATTCTCGGGTGCCAAGG1756660.4082080809472725Illumina Small RNA Adapter 2 (100% over 21bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA1321200.30701701897210415RNA PCR Primer, Index 1 (100% over 22bp)
TTGTTTCGCAAAAGTTGACCGAACTTGTGGAATTCTCGGGTGCCAAGGAA1177580.2736429769915004RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG1164810.27067551761194114No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC1079000.2507352130418562No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATG1051890.24443546176700473RNA PCR Primer, Index 8 (100% over 49bp)
TAAATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAA1037410.24107063703591472No Hit
GGTTGTTTCGCAAAAGTTGACCGAACTTGTGGAATTCTCGGGTGCCAAGG1025730.2383564690207814Illumina Small RNA Adapter 2 (100% over 21bp)
TACGGTTGTTTCGCAAAAGTTGACCGAACTTGTGGAATTCTCGGGTGCCA1012730.23533556283955423No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA872570.20276554665795407No Hit
AAATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAG764190.1775804842024616No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCCA750920.17449683612362432No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG719310.16715138655527115No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTG713090.1657059991362532No Hit
AATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAGG707090.1643117347449176Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG689610.1602497778181598Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA655720.1523745077810991RNA PCR Primer, Index 1 (100% over 22bp)
ATAGTTTATTCCAATTAAATTGCTTGCATTTGGAATTCTCGGGTGCCAAG654980.1522025485061677No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCC637970.1482498089567312No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT620500.144190175803959RNA PCR Primer, Index 1 (100% over 25bp)
ACCCAGAAGGCCCGGGTTCGATTCCCGGTATGGGTGGAATTCTCGGGTGC617530.14350001493024786No Hit
CCTCGATAGTATAGTGGTTAGTATCCCTGGAATTCTCGGGTGCCAAGGAA556100.12922507133695663RNA PCR Primer, Index 1 (100% over 23bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA546560.127008190954733RNA PCR Primer, Index 1 (100% over 22bp)
TGCATTTTAACACAGAATAAATGTTATTTGGAATTCTCGGGTGCCAAGGA534850.12428705161764297RNA PCR Primer, Index 1 (100% over 22bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG529850.1231251646248633No Hit
TGTTTCGCAAAAGTTGACCGAACTTGTGGAATTCTCGGGTGCCAAGGAAC515830.11986723349710907RNA PCR Primer, Index 1 (100% over 24bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA511880.11894934277281309No Hit
CCCATATTGTCTAGTGGTTAGGATATCCTGGAATTCTCGGGTGCCAAGGA485570.11283549341680639RNA PCR Primer, Index 1 (100% over 22bp)
GCATCGGTGGTTCAGTGGTAGAATGCTTGGAATTCTCGGGTGCCAAGGAA474980.11037461676609903RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGTGGAATTCTCGGGTGCCAAGG465500.10817167902778874Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAA461360.10720963659776717No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGTGGAATTCTCGGGTGCCAAG454020.10550398649236657No Hit
ATTTATCATAAAATATAACTTATAGTTTATTCTGGAATTCTCGGGTGCCA433970.10084481965132004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)