FastQCFastQC Report
Sun 2 Jul 2023
SRR3031130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44738296
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG24463905.468223465641159No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGTGGAATTCTCGGGTGCCAAGG6033451.348609701183076Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA5168101.1551848107938667RNA PCR Primer, Index 1 (100% over 22bp)
TATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAGG3836410.8575226021125167Illumina Small RNA Adapter 2 (100% over 21bp)
AATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAGG3655810.817154502263564Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG3470260.7756799677841999Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA3321630.7424578709926726RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCCA2874160.6424384156249492No Hit
TTATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAG2203300.49248634771427147No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA2065230.46162464480095533RNA PCR Primer, Index 1 (100% over 22bp)
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA1864460.4167481032357602RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCTGGAATTCTCGGGTGCCAAGG1829840.4090097664873065Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG1755330.3923551312727691No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG1703290.3807230387138571Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAGG1647360.36822144500094506Illumina Small RNA Adapter 2 (100% over 21bp)
GAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAAGGA1562770.3493137065390242RNA PCR Primer, Index 1 (100% over 22bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA1463900.32721407180997686RNA PCR Primer, Index 1 (100% over 22bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1445560.3231146756237654Illumina Small RNA Adapter 2 (100% over 21bp)
AATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAG1402430.3134741653995941No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1378510.30812751563000973No Hit
TGCATTTTAACACAGAATAAATGTTATTTGGAATTCTCGGGTGCCAAGGA1377130.3078190550663798RNA PCR Primer, Index 1 (100% over 22bp)
ATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAGGA1265450.28285610162711605RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATTGTCTAGTGGTTAGGATATCCTGGAATTCTCGGGTGCCAAGG1225700.2739710962616904Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1152880.2576942134765258RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCC1143090.2555059316519342No Hit
ATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAGGA1135000.2536976374781909RNA PCR Primer, Index 1 (100% over 22bp)
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC1098610.24556366652855977No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG1075210.24033324827570546No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG953640.2131596607970943No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG884040.19760251932706602Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTATCATAAAATATAACTTATAGTTTATTCTGGAATTCTCGGGTGCCA873530.19525330155623272No Hit
TATGGCTCTCTCAGTCGCTTCCCGGGAATGGAATTCTCGGGTGCCAAGGA871970.19490460700604242RNA PCR Primer, Index 1 (100% over 22bp)
TTATGGCTCTCTCAGTCGCTTCCCGGGAATGGAATTCTCGGGTGCCAAGG839670.187684841639923Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG839050.1875462579084371Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG810920.18125857989763403Illumina Small RNA Adapter 2 (100% over 21bp)
AATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGGA799150.1786277242208778RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGATAGTATAGTGGTTAGTATCCCTGGAATTCTCGGGTGCCAAGGA789740.17652438081235816RNA PCR Primer, Index 1 (100% over 22bp)
TTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAG744020.166304948226012No Hit
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC741490.16573943719269058RNA PCR Primer, Index 1 (100% over 24bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGATTGGAATTCTCGGGTGCCAAG735200.16433348288455152No Hit
TGGTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAA722240.16143663585220142No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG706160.15784239971947078No Hit
TCGCAGAGAGATATGGTAGATGGGCTAGTGGAATTCTCGGGTGCCAAGGA631600.14117658839755542RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGGTAGCTCAGTCGGTAGAGCATTGTGGAATTCTCGGGTGCCAAGG604000.135007377124958Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATTGTCTAGTGGTTAGGATACCCTGGAATTCTCGGGTGCCAAGG589070.13167019146191888Illumina Small RNA Adapter 2 (100% over 21bp)
TACTGCGATCCACTGAAGCTTATCCTTTTGGAATTCTCGGGTGCCAAGGA587610.1313438491264844RNA PCR Primer, Index 1 (100% over 22bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA572850.12804466222853012No Hit
CTGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAAG562840.12580720553147576No Hit
TTAGTGAGGTCTCCGGACGTGATCACTGTTGGAATTCTCGGGTGCCAAGG556310.12434760590792282Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAGG535620.11972293267495032Illumina Small RNA Adapter 2 (100% over 21bp)
TGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAAGG527010.11779840698447701Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCTCTCTCAGTCGCTTCCCGGGAATGGAATTCTCGGGTGCCAAGGAA511520.11433604891880549RNA PCR Primer, Index 1 (100% over 23bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA494820.11060322905458893No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATTGGAATTCTCGGGTGCCAAGGAA491100.10977172666567364RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA488790.10925539050481493No Hit
ACTGGAACGTACCTTGAGCATATATGCTGTGGAATTCTCGGGTGCCAAGG463150.10352428264143096Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA463050.10350193042667516No Hit
GGGGATATAGCTCAGTGGTAGAGCATTCTGGAATTCTCGGGTGCCAAGGA461800.10322252774222782RNA PCR Primer, Index 1 (100% over 22bp)
ACTGGAACGTACCTTGAGCATATATGCTGTTGGAATTCTCGGGTGCCAAG447820.1000976881193687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)