FastQCFastQC Report
Sun 2 Jul 2023
SRR3031131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43027179
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG6801361.5807125073200825No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA6791071.5783209956664834RNA PCR Primer, Index 1 (100% over 22bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA4826921.1218304597659075RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG4230260.983159969655459Illumina Small RNA Adapter 2 (100% over 21bp)
TAATTTTTCGGAATTATATAATGGCATATGGAATTCTCGGGTGCCAAGGA4012080.9324524854394939RNA PCR Primer, Index 1 (100% over 22bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA3933950.9142941953038566RNA PCR Primer, Index 1 (100% over 22bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG3504280.8144340580636252Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC3345580.7775503943681736RNA PCR Primer, Index 1 (100% over 24bp)
AATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAGG2991600.6952814638394025Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG2861150.6649634176574765Illumina Small RNA Adapter 2 (100% over 21bp)
TGCATTTTAACACAGAATAAATGTTATTTGGAATTCTCGGGTGCCAAGGA2832520.6583094838729725RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCCA2824780.6565106208798862No Hit
ATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAGG2686870.6244587868519105Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGTGGAATTCTCGGGTGCCAAGG2532650.5886163255090462Illumina Small RNA Adapter 2 (100% over 21bp)
ATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAGGA2288920.5319707341259812RNA PCR Primer, Index 1 (100% over 22bp)
TGCATTTTAACACAGAATAAATGTTATTGGAATTCTCGGGTGCCAAGGAA2204670.5123900872051128RNA PCR Primer, Index 1 (100% over 23bp)
AATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGGA2156360.5011623002288855RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCTGGAATTCTCGGGTGCCAAGG2109610.4902970747861486Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA1970770.4580290983055152RNA PCR Primer, Index 1 (100% over 22bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA1927810.448044711460168RNA PCR Primer, Index 1 (100% over 22bp)
AATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGGAA1833900.426218971966533RNA PCR Primer, Index 1 (100% over 23bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1707460.3968328948546685RNA PCR Primer, Index 1 (100% over 22bp)
TATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAGG1577640.36666126775357505Illumina Small RNA Adapter 2 (100% over 21bp)
AATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAG1535430.3568511893377904No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCTGGAATTCTCGGGTGCCAAGGA1453970.33791896977489505RNA PCR Primer, Index 1 (100% over 22bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG1341470.31177270534050117No Hit
ATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAGGA1321780.3071965280363837RNA PCR Primer, Index 1 (100% over 22bp)
TTATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAG1180570.27437773691833245No Hit
ATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGGAA1158850.2693297647981988RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1116600.2595103899328376RNA PCR Primer, Index 12 (100% over 50bp)
TTAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGG1115010.2591408560621648Illumina Small RNA Adapter 2 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG1079350.25085307126456047No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCTGGAATTCTCGGGTGCCAAGG1061750.2467626334508242Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAG1003970.23333391203731949No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG947800.2202793727192759Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCC915220.2127074145390754No Hit
TAATTTTTCGGAATTATATAATGGCATTGGAATTCTCGGGTGCCAAGGAA873520.20301586585539338RNA PCR Primer, Index 1 (100% over 23bp)
TTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAGGAAC839820.19518360708704607RNA PCR Primer, Index 1 (100% over 24bp)
TTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAGGA838380.19484893490228583RNA PCR Primer, Index 1 (100% over 22bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG836840.19449102159358392Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG823770.1914534066944059Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCATTTTAACACAGAATAAATGTTATTGGAATTCTCGGGTGCCAAGGA744790.17309756700526427RNA PCR Primer, Index 1 (100% over 22bp)
AGTCACTTAGCGATGTACACTGACTGTGTGGAATTCTCGGGTGCCAAGGA704330.16369420825845915RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATTGTCTAGTGGTTAGGATACCCTGGAATTCTCGGGTGCCAAGG670520.15583638425377597Illumina Small RNA Adapter 2 (100% over 21bp)
TTAATTTGATAAAGTGCTGATAGATTTATGGAATTCTCGGGTGCCAAGGA670480.1558270878041993RNA PCR Primer, Index 1 (100% over 22bp)
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC665560.1546836245062685No Hit
ATTAATTTGATAAAGTGCTGATAGATTTATGGAATTCTCGGGTGCCAAGG621050.14433900023982515Illumina Small RNA Adapter 2 (100% over 21bp)
TAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGGA612000.14223567852310282RNA PCR Primer, Index 1 (100% over 22bp)
ATTTATCATAAAATATAACTTATAGTTTATTCTGGAATTCTCGGGTGCCA589680.13704825965931905No Hit
TGCATGGGATAATGAAATAAGACCTCTGTGGAATTCTCGGGTGCCAAGGA587150.13646025922359448RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGATAGTATAGTGGTTAGTATCCTGGAATTCTCGGGTGCCAAGGAA579380.13465442389332566RNA PCR Primer, Index 1 (100% over 23bp)
TTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGAA560320.1302246656700408RNA PCR Primer, Index 1 (100% over 23bp)
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA559110.1299434480703464RNA PCR Primer, Index 1 (100% over 22bp)
TTGCATTTTAACACAGAATAAATGTTATTTGGAATTCTCGGGTGCCAAGG519210.12067023961761472Illumina Small RNA Adapter 2 (100% over 21bp)
TGCACTTGTATGATTAACAATGCGAAAGTGGAATTCTCGGGTGCCAAGGA518420.12048663473847541RNA PCR Primer, Index 1 (100% over 22bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACTGGAATTCTCGGGTGCCAA517390.12024725116187608No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA515340.11977080812107158RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA515050.11970340886164067RNA PCR Primer, Index 1 (100% over 23bp)
ATTAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAG513750.11940127425039879No Hit
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC478990.11132265956826963RNA PCR Primer, Index 1 (100% over 33bp)
TAATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGG477580.1109949597206919Illumina Small RNA Adapter 2 (100% over 21bp)
TTAATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAG457740.1063839207306619No Hit
ATAATTTTGAACGAAAGGGAATACGGTTTGGAATTCTCGGGTGCCAAGGA452500.10516608583611767RNA PCR Primer, Index 1 (100% over 22bp)
TACTGCGATCCACTGAAGCTTATCCTTTTGGAATTCTCGGGTGCCAAGGA450860.10478493140347407RNA PCR Primer, Index 1 (100% over 22bp)
ATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGAAC445290.10349040079992229RNA PCR Primer, Index 1 (100% over 24bp)
TCGGCTGAGTTGACCTCTGCGATTATTCCTTGGAATTCTCGGGTGCCAAG438730.10196578306934787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)