FastQCFastQC Report
Sun 2 Jul 2023
SRR3031133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45609310
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG35395917.760676493461532No Hit
TGGTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAA16677693.6566415935693835No Hit
TGGTTTATGTGCTCGTTCTGGGTAAATAGAGTTGGAATTCTCGGGTGCCA6277881.3764470455703013No Hit
AATACTTGTATTTTTTCATATGTTCCTCCTTGGAATTCTCGGGTGCCAAG5916061.2971167509440507No Hit
GTGCTGAAGCTTATGTAGCCTTCATTCATGTGGAATTCTCGGGTGCCAAG4423110.9697822659452642No Hit
ATACTTGTATTTTTTCATATGTTCCTCCTTGGAATTCTCGGGTGCCAAGG2511770.5507143168796019Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGA2436960.5343119639389414RNA PCR Primer, Index 1 (100% over 22bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA2362670.5180236228085888RNA PCR Primer, Index 1 (100% over 22bp)
AATACTTGTATTTTTTCATATGTTCCTCCTGGAATTCTCGGGTGCCAAGG2325180.5098038097923429Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG1870940.410210108418654RNA PCR Primer, Index 2 (100% over 49bp)
ATGGTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCA1633620.35817687222192135No Hit
GTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAAGG1434430.3145037712695062Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTTATGTTAGTTACTTGTTCCCCGGTGGAATTCTCGGGTGCCAAGGA1320280.2894759863720806RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1279070.28044055040516946RNA PCR Primer, Index 2 (100% over 50bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG1226930.26900867388697614No Hit
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA1225500.2686951414086291RNA PCR Primer, Index 1 (100% over 23bp)
GTTTATGTGCTCGTTCTGGGTAAATAGAGTTGGAATTCTCGGGTGCCAAG1213420.2660465593537811No Hit
TGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAG1165030.2554368833906937No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGTGGAATTCTCGGGTGCCAAGG1051150.23046829693323576Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAAGGAACT1008630.22114563890574096RNA PCR Primer, Index 1 (100% over 25bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG946200.20745764406433684Illumina Small RNA Adapter 2 (100% over 21bp)
TGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT917800.20123084519366768RNA PCR Primer, Index 2 (100% over 46bp)
AGCATTTATGTTAGTTACTTGTTCCCCGGTGGAATTCTCGGGTGCCAAGG828930.18174578830506316Illumina Small RNA Adapter 2 (100% over 21bp)
GTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTA822090.18024609449254989RNA PCR Primer, Index 2 (100% over 47bp)
CTGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAAG819130.17959710418771957No Hit
TATGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCA819000.17956860123514257No Hit
ATGGTGCTGAAGCTTATGTAGCCTTCATTCTGGAATTCTCGGGTGCCAAG807000.17693755945880346No Hit
CATATGTTCCTCCTATTTAAAAACCTGCATGGAATTCTCGGGTGCCAAGG787490.17265992403743885Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCTGGAATTCTCGGGTGCCAAGG774800.16987759735896027Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA766590.1680775262769816RNA PCR Primer, Index 1 (100% over 22bp)
ATACTTGTATCTTTTCATATGTTCCTCCTTGGAATTCTCGGGTGCCAAGG706120.15481926825904624Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA690560.15140768408905989RNA PCR Primer, Index 1 (100% over 22bp)
GGTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAAG647670.14200390227346127No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTG645730.1415785505196198No Hit
TTATAGTTTATTCCAATTAAATCGCTTGCTGGAATTCTCGGGTGCCAAGG619200.1357617556590968Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA598260.1311705877593851No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG594860.13042512592275568Illumina Small RNA Adapter 2 (100% over 21bp)
TATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTAT581420.12747835913325592RNA PCR Primer, Index 2 (100% over 48bp)
TTATGTTAGTTACTTGTTCCCCGGATAGTGGAATTCTCGGGTGCCAAGGA570080.12499202465461547RNA PCR Primer, Index 1 (100% over 22bp)
TACTTGTATTTTTTCATATGTTCCTCCTTGGAATTCTCGGGTGCCAAGGA560860.12297050755646162RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG559530.12267890042625068No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA544800.11944929664579446No Hit
GGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT534970.11729403492401003RNA PCR Primer, Index 2 (100% over 43bp)
TCGCAGAGAGATATGGTAGATGGGCTAGTGGAATTCTCGGGTGCCAAGGA522450.11454898133736292RNA PCR Primer, Index 1 (100% over 22bp)
CATATGTTCCTCCTATTTAAAAACCTGCATTGGAATTCTCGGGTGCCAAG512920.11245949565998696No Hit
CATATGTTCCTCCTATTTAAAAACCTGCATTATGGAATTCTCGGGTGCCA512100.11227970780527045No Hit
ATGTATGTAAGCGTATTACCGGTGGAGTTCTGGAATTCTCGGGTGCCAAG468120.10263693969498772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)