FastQCFastQC Report
Sun 2 Jul 2023
SRR3031134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40163347
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG14711463.6629068787519126No Hit
AATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAG2869710.7145096747041525No Hit
AATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGGA2028200.5049877939704577RNA PCR Primer, Index 1 (100% over 22bp)
ATTTGATAAAGTGCTGATAGATTTATATGTGGAATTCTCGGGTGCCAAGG1564610.38956165680116256Illumina Small RNA Adapter 2 (100% over 21bp)
AATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGGAA1519770.378397248615759RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA1367930.3405916344571581No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1311470.3265340410997121RNA PCR Primer, Index 1 (100% over 22bp)
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA1246450.3103451512644103RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG1204490.2998978147911826No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG1062690.26459199229586117Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC980250.24406581453482948RNA PCR Primer, Index 14 (100% over 50bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA926910.23078504886557388RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA899800.22403511340825258RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGTGGAATTCTCGGGTGCCAAGG892980.22233704775650298Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG826030.20566762028075003Illumina Small RNA Adapter 2 (100% over 21bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCTGGAATTCTCGGGTGCCAAGGA803060.1999484754096814RNA PCR Primer, Index 1 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG748450.1863515010340149No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA739030.18400607897543997No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG703050.1750476622379106No Hit
ATTAATTTGATAAAGTGCTGATAGATTTATGGAATTCTCGGGTGCCAAGG657430.1636890471304595Illumina Small RNA Adapter 2 (100% over 21bp)
TTAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGG582810.14510991825457176Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAAGG582220.1449630181468691Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGGAATTCTCGGGTGCCA521310.1297974494008181No Hit
TTAATTTGATAAAGTGCTGATAGATTTATGGAATTCTCGGGTGCCAAGGA513720.12790766665935488RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCCA505590.12588343297185867No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA502310.12506676796632513No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG501240.12480035590659315No Hit
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC468440.11663370585125786RNA PCR Primer, Index 1 (100% over 27bp)
ATTTGATAAAGTGCTGATAGATTTATATGGAATTCTCGGGTGCCAAGGAA456200.11358615107451081RNA PCR Primer, Index 1 (100% over 23bp)
TGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAG447580.11143991560265135No Hit
ATTAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAG441260.10986634156759895No Hit
TGCATTTTAACACAGAATAAATGTTATTTGGAATTCTCGGGTGCCAAGGA438910.10928123096912218RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATTGTCTAGTGGTTAGGATATCCTGGAATTCTCGGGTGCCAAGG438440.10916420884942682Illumina Small RNA Adapter 2 (100% over 21bp)
TAATTTGATAAAGTGCTGATAGATTTATTGGAATTCTCGGGTGCCAAGGA429390.10691091058720778RNA PCR Primer, Index 1 (100% over 22bp)
AGTCACTTAGCGATGTACACTGACTGTGTGGAATTCTCGGGTGCCAAGGA418120.10410486954685325RNA PCR Primer, Index 1 (100% over 22bp)
TCGCAGAGAGATATGGTAGATGGGCTAGTGGAATTCTCGGGTGCCAAGGA405010.10084069935705309RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)