FastQCFastQC Report
Sun 2 Jul 2023
SRR3031135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3031135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33371505
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG335342710.0487736468583No Hit
ATGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA16307804.886743945171188No Hit
AGAAACGGATAGAGCGAATTCTGAGTGGTTGGAATTCTCGGGTGCCAAGG4452211.3341352150584758Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGTGCTGAAGCTTATGTAGCCTTCATTCTGGAATTCTCGGGTGCCAAG2776470.8319882486570503No Hit
AAGCCTAGAATCAATTGTAAACGACTTTTGTTGGAATTCTCGGGTGCCAA2058980.6169874568138296No Hit
TGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAG1850810.5546078907738803No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG1800990.5396789866084853RNA PCR Primer, Index 16 (100% over 49bp)
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG1777770.5327209545988411Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCTAGAATCAATTGTAAACGACTTTTGTTGGAATTCTCGGGTGCCAAG1467930.4398752768267419No Hit
TCGGGACGCAGGGCTTCGATGATCGCTCGGTTGGAATTCTCGGGTGCCAA1236160.37042380917492335No Hit
ATGTTAGTTACTTGTTCCCCGGATAGTTTTGGAATTCTCGGGTGCCAAGG1188600.3561721294859192Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA1134590.33998766312756945No Hit
TCGGGACGCAGGGCTTCGATGATCGCTCGGTGGAATTCTCGGGTGCCAAG1132820.3394572705066793No Hit
CAGAGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTC1099330.3294217626684802RNA PCR Primer, Index 1 (100% over 26bp)
TTATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAG1095480.32826808380383204No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1069340.3204350537981431No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTTGGAATTCTCGGGTGCCA962040.2882818740119752No Hit
ATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAGGAA876010.2625023953819284RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA859990.25770189267760024RNA PCR Primer, Index 1 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG782120.2343676139269116No Hit
ATGGTGCTGAAGCTTATGTAGCCTTCATTCATGGAATTCTCGGGTGCCAA744240.22301661252616564No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG684930.20524396487362498Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTCC639150.19152567437399062RNA PCR Primer, Index 1 (100% over 27bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG623930.18696489714803094No Hit
ACGATTATCAATTAACAATCAATTCAGAACTTGGAATTCTCGGGTGCCAA592720.1776126069231819No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA587850.176153278073614RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA567920.17018111709376008No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC549590.16468840707064306RNA PCR Primer, Index 16 (100% over 50bp)
CGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG506750.15185110770401275No Hit
ATGTATTTTTGATTTGAAAATTTGGTATTGGAATTCTCGGGTGCCAAGGA501920.15040376512836323RNA PCR Primer, Index 1 (100% over 22bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG498800.14946883576272632Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG479380.14364949977533228No Hit
TTGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA442170.13249926846271992No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA442090.13247529591488308RNA PCR Primer, Index 1 (100% over 23bp)
GTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTA387930.11624588102933926RNA PCR Primer, Index 16 (100% over 47bp)
AGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG387660.1161649736803899No Hit
ATGTATGTAAGCGTATTACCGGTGGAGTTCTGGAATTCTCGGGTGCCAAG365890.10964144410028855No Hit
TTATAAGCATATGGTATATTATTGGATAAATTGGAATTCTCGGGTGCCAA354910.10635121190968164No Hit
GAAGCTTATGTAGCCTTCATTCATGTTGGCAGTGGAATTCTCGGGTGCCA351970.10547022077667759No Hit
AAACGGATAGAGCGAATTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAA349240.10465215758174526RNA PCR Primer, Index 1 (100% over 23bp)
CGGGACGCAGGGCTTCGATGATCGCTCGGTTGGAATTCTCGGGTGCCAAG345730.10360036204540371No Hit
ATGGTGCTGAAGCTTATGTAGCCTTCATTCATTGGAATTCTCGGGTGCCA334720.10030114014935795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)