FastQCFastQC Report
Thu 28 Sept 2023
SRR3129148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92495359
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1620293717.517567557092242Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC53596035.794456130496234No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG24109782.606593483247089No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT17874971.9325261497714714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG17435801.8850459297098356No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT14449451.5621810819719073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC11398611.2323439925239925No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA10389341.123228247592401No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT9927551.0733024994259441No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC9579641.0356887203389307No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT7052700.7624923105601439Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA6461670.6985939694552675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT6166440.6666756112595876Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC5198590.5620379288435433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC4950890.5352582068468971No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC4548990.49180737814099407Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA3986770.43102378790702356Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA3967350.4289242231061561No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3832210.41431376032607214No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACG3774700.4080961510728338No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA3386570.3661340457092555No Hit
CGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAG3334430.3604970061254641Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC3277430.35433453477379334No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA3171190.342848553082539No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC3079140.33289670241725317No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2902900.3138427734520172No Hit
AGCGGCCCCCGCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT2866560.3099139276814959No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2850500.3081776243497795No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2565280.27734148261427904No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2545110.2751608326640475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACGTCTGAACTCCA2534500.27401374808437684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2524460.2729282882182229Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA2463770.26636687793168085Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTCTG2376540.2569361344929749Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGCCCCCGGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTG2363660.25554363219456233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCTGAACTCC2313170.25008497993937184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCT2088880.22583619573820998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA2009190.21722062833444433Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGCCCCCGGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1989180.21505727654941043No Hit
GCGACCTCAGATCAGACGTGGCGACAGATCGGAAGAGCACACGTCTGAAC1914250.20695632956027557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT1839080.19882943532334418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGAA1831540.19801425928840385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAACTCC1698670.18364921422706193Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1653260.17873977871689756Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1608970.17395143036311692No Hit
ACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA1560580.16871981652614593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1547120.16726460837889176No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA1513140.1635909105450361Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1493600.16147837212027039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC1492780.1613897190236323No Hit
CGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCACACGTCTGAACTCC1412450.152704958959076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1350840.1460440842226473Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG1332470.1440580386308896No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCAC1291830.13966430467068083No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTGAA1285840.13901670461109297Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC1261070.13633873241142833No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA1194340.12912431638867417Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1191830.12885295142213568No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1178520.12741396030475433No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1142240.12349160134618214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG1140170.12326780633393725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1127230.12186881722357551Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGT1106640.11964275958970007No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC1102150.11915732982884038No Hit
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC1088180.11764698378001863Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTAAATACCGGCACGAGACCGAGATCGGAAGAGCACACGTCTGAAC1049290.11344244850165941Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1006690.10883681201777919No Hit
CCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACTCC1001960.10832543500912299Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA973390.10523663138601366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC946620.1023424321213781Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCA944300.10209160872601186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAA943090.10196079135170447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGCCCCCGCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACG941230.10175970018128154No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGATCGGAAGAGCACACGTCTG930720.10062342695485943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)