FastQCFastQC Report
Thu 28 Sept 2023
SRR3129149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77831110
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1099657414.128764192107756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC41576475.341883213537621No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG17378442.2328397988927566No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG13167131.691756676732479No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT12663251.627016497644708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT10513771.3508441547345271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC8746481.1237768547821043No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA8009361.0290692243757027No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT7654520.983478200426539No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC7045760.9052626899449334No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG5585390.7176294928853Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT5357310.6883250155368464Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA4553460.5850436926827846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT4214070.5414377361443259Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC3711080.47681190721807776Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC3551210.4562712776420637No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC3421850.4396506743897138Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3316280.4260866894998671No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA3072870.39481256274001486No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACG2866940.36835399109687633No Hit
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA2812140.3613131047469322Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2536850.32594292950466724No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA2517530.32346063161632926No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2475810.31810030719078786No Hit
CGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAG2339560.30059445381159283Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC2310490.296859443479606No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2303750.29599346585189396No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2229530.28645743328085643No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2136140.27445837532061407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGCCCCCGCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT2064270.26522427856932784No Hit
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1929200.24787003551664627Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1846850.23728943349259699No Hit
AGCGGCCCCCGGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTG1748590.22466466172716798Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACGTCTGAACTCCA1710780.21980670711236164Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTCTG1704660.2190203891477328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1649210.2118959886348788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1631610.20963468206993321Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCTGAACTCC1587180.2039261678267212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCT1501820.19295883098673525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA1444400.18558131831860036Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGCCCCCGGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1441440.18520100766904135No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1432330.18403052455502691No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA1384590.17789673049761207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACAGATCGGAAGAGCACACGTCTGAAC1333900.17138391062391375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT1318820.1694463820444036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1295070.16639490301500262TruSeq Adapter, Index 6 (100% over 49bp)
AAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGAA1284920.16509079724033232Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1258020.1616345957291371No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1201760.15440612372096454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1188690.15272684662983735No Hit
AGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAACTCC1180780.15171054350888738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC1162140.14931561428328596No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1149410.14768002152352702No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA1136730.1460508529301458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA1044050.13414301813246657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG1029410.13226202221708003No Hit
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1027500.13201661906145243Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCAC1026300.13186243906838796No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1025130.13171211357515009Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTGAA1013310.13019344064346505Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA1009170.12966151966739264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1007170.12940455301228518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC997350.1281428467357076No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA987180.1268361712944862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCACACGTCTGAACTCC984670.1265136781423264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA974070.1251517548702569Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA901760.11586112545484703No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC893190.11476002333771161Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG866120.11128197966083228No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC847340.10886906276937332No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG815310.10475374178782752Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGT806310.103597391839844No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA793630.10196822324646276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)