FastQCFastQC Report
Thu 28 Sept 2023
SRR3129152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51846799
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC30396455.862743811821439Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC20981994.046921006637266No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT17300273.3368058074327793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC14804232.8553797506380287No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT11815402.278906360255722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG10006921.9300940835325242No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT9182491.771081373798988No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT9162561.767237356350582No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG8362561.6129366057873697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT8189771.5796095724250983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC6218651.1994279531123995Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA6109131.1783041803602958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA5937821.1452626033865658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC5206911.0042876513938692No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3002300.5790714292699151No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2870370.5536253067426593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC2784810.5371228414699237No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2699270.5206242337159522No Hit
AAGGAGTCTAACACGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC2671500.5152680689120268Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA2604140.5022759457146043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2558220.4934190826322759No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC2450570.47265598788461366No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACG2250680.4341020165970131No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2124910.409844009849094No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC2111170.4071938944581709No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT1937880.3737704231267971No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA1918050.36994569327221144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTCTGAACT1917940.369924476919009Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC1881060.362811212318045Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTGAA1856430.35806067796008Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1770540.34149456362773717No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCACACGTCTG1690670.3260895624433825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1653570.3189338651360135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA1609400.31041453494554216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1509300.29110765353132023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1202460.23192560065280016Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1089990.2102328438829946No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1047870.20210890936584147No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1012120.19521359457504792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC905300.174610586856095No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT891790.17200483293095878No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGAGATCGGAAGAGCACACGTCT875010.16876837468789538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT851520.16423771889948308No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA823450.15882369131409638No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGAGATCGGAAGAGCACACGTCTGA821040.1583588603030247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAGCGCACGTGTTAGGACCCGAAGATCGGAAGAGCACACGTCTGAACT807640.1557743227310909Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACAGATCGGAAGAGCACACGTCTGAAC805890.15543678983923384Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGAGTCTAACACGTGCGCGAGTCGGGGAGATCGGAAGAGCACACGTCTG796590.15364304361393652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG788920.15216368516791173No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA769140.1483485991102363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAAC760110.14660692938825404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAA731270.14104438733045024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG725480.13992763564824898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG714950.1378966520189607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT639030.12325351079051187No Hit
ACGAGCGCACGTGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTGAA636730.12280989613264263Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGAGCGCACGTGTTAGGACCCGAAAGATAGATCGGAAGAGCACACGTCT600920.11590300878555684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGA595260.11481133097532212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGATTAAGAGGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAAC591300.11404754226003423Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA590160.11382766369048164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT575760.11105025018034381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG573360.1105873479286542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA572430.11040797330612445Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA543220.10477406715118517No Hit
CAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAAC542060.1045503310628685Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGGCCCCCGCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT541460.1044346054999461No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC531650.10254249254616471Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)