FastQCFastQC Report
Thu 28 Sept 2023
SRR3129153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47674727
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC27935085.859515461934371Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC17380313.6456024173982158No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT14482773.037829666019902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC11588452.4307323249066535No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT10001562.097874624431515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG8002441.6785497271961305No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT7894631.6559360686008753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7515181.5763446322408936No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT7291941.5295189839262215No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG7072551.4835008913632584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC5548851.1638975929531803Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA5308491.1134809434776627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA5135811.0772604948529647Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4377920.9182894744211121No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA2690100.564261227966759Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGAGTCTAACACGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC2538400.5324414338020226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2434510.5106500137903254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2369420.496997077717928No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC2311360.4848187174726769No Hit
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA2286500.4796042146187853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2095740.4395914002821663No Hit
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC2066550.4334686594010281Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2051080.4302237535623434No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC2027970.4253763215046832No Hit
AAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTCTGAACT1892850.39703426094081257Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACACG1765520.3703261898070229No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1731520.3631945286231005No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTGAA1692840.35508121525268516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1688210.3541100508032275No Hit
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA1469530.30824088410616385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCACACGTCTG1468450.30801434898620395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT1446450.30339974469072467No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1419040.297650367248039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1382100.28990202712644797No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1273320.2670849064327101Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1081550.22686023980798045Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1061240.2226001210242903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT884540.18553645834196386No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG834560.17505291640159787No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG824440.17293019842567742TruSeq Adapter, Index 8 (100% over 49bp)
ACGAGCGCACGTGTTAGGACCCGAAGATCGGAAGAGCACACGTCTGAACT798590.1675080383784893Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACAGATCGGAAGAGCACACGTCTGAAC761610.1597513080672701Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGTCTAACACGTGCGCGAGTCGGGGAGATCGGAAGAGCACACGTCT731920.15352368981578018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAAGAGCCCAGCGCCGAATCCCCGAGATCGGAAGAGCACACGTCTGA725570.1521917472123123Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT721950.15143243505096526No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT709760.14887552476178834No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC698260.14646334524369692No Hit
AGGAGTCTAACACGTGCGCGAGTCGGGGAGATCGGAAGAGCACACGTCTG690940.14492794054174657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAAC680260.1426877599110321Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA658010.13802071693037696Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAA653720.13712086909275853Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA650200.13638253240548184No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG634100.13300548108015386No Hit
AAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG630990.13235314383656566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG618720.12977945316813244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC591470.12406363648395931Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGATTAAGAGGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAAC552820.1159566157557651Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAGCGCACGTGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTGAA548900.115134377172207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT523610.1098296797798129No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA512410.10748042668393255Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA511800.10735247629210336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGA507500.10645053090707789Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAGCGCACGTGTTAGGACCCGAAAGATAGATCGGAAGAGCACACGTCT502510.1054038547509669Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG499060.10468020089553946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAAC497000.10424810612969006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT479940.1006696902532866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)