FastQCFastQC Report
Thu 28 Sept 2023
SRR3131003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3131003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9593373
Total Bases489.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2894203.016874252674216No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2401502.503290552759702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2396652.4982349795009533No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1336581.393232599211977Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1106151.153035538178282Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1093501.1398493522559792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1053031.0976639811669993No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1049031.0934944362113306No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1017711.0608468992084432No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA976651.018046520238502No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA952470.9928416209814838No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA838690.8742389147174826No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT739620.7709697100279537No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG730790.7617654395383147TruSeq Adapter, Index 7 (100% over 49bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA724420.755125439196412No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG695020.7244792837722457No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA582910.6076173625272362No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC554720.5782324944521599No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA511670.5333577668667736No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG500640.5218602466515166No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC483210.5036914545071894No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC461720.4812905742328584No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT447260.46621766921811547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT417920.4356340569682843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC406340.4235632243216229Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC405690.42288567326632664No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT388910.40539443217729576No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA375340.3912492509151891Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC367680.38326457232508315No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG354360.3693799876227058No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC351050.36592968917188984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG306450.31943926291618185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGA289140.30139555712052474No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA285190.29727813147680177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC273600.2851968749677512Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA265540.2767952418820784No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG249020.25957502121516596No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT247680.25817822365501686No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC233560.24345972996150572No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA232880.24275090731904203No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG225990.23556886613290234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA224860.23439096968292591No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG184770.19260170536473462No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC179150.1867434947020198No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC177160.18466914608657456Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC173310.18065595906674325No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG170850.1780916889190069No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA165610.17262958502708067No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG160220.16701112319931685No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT152790.1592661934441619No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT150200.1565664130853663No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC145910.15209457612041147Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAA140410.14636145180636675No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG140100.1460383120723024No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC138650.14452685202587243No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC138450.144318374778089No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAGC134340.14003416733613924No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC134300.13999247188658254No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA132260.1378660039591914No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT129850.1353538531234009No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT128480.13392578397608432Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT128430.13387366466413847Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC126830.1322058466818709No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC125780.13111134113100784No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT124040.12929758907529187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC122000.12717112114790075No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG121820.12698349162489567No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG117860.12285564211878346No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC116290.12121909572368342No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT110710.11540258051052535No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAG105520.10999259593054496No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG103640.1080329098013806No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT102640.10699052356246339No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA101900.10621915774566464Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC101150.10543736806647673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA100850.10512465219480156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT98700.10288352178112953No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT97660.10179944009265562No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACA97540.10167435374398558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)