FastQCFastQC Report
Thu 28 Sept 2023
SRR3131015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3131015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14149142
Total Bases721.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10422907.36645373973913No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT4569313.2293901637286555No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4462933.154205392807564No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG4192172.962843966086424No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3246992.2948317290193287No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2382031.6835155092796439Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2069611.4627106011092403TruSeq Adapter, Index 12 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1776271.2553906095507417No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1395920.9865757231074506No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1371140.9690622936712346No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1277210.9026766428664015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA887530.6272677170106852No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA748730.5291698959555285No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT714670.5050977649386796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA706400.4992528875602492No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC694070.49053857824029184No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC655530.4633001774948615No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT648590.4583952864421037No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA644500.45550465180150146No Hit
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC642270.4539285845035692Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC638170.45103088229660854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC630480.4455959237669676No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT600350.4243013463289859Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC588510.4159333477605921Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT562730.39771316168853205No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG530560.3749768007134284No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA483200.34150480643985337No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC442130.31247831140573756No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG439810.3108386360105793No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC418680.2959048682951942No Hit
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC385500.272454683117888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGTCTGAACTC349270.24684889020125744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT346290.2447427554264421No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC332820.23522274354162254No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA323580.22869231222642333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC321180.22699609630039758No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT313940.22187917825688655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC305590.21597776034758853No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT298720.21112234225933985Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG292090.20643654576369364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG280350.1981392228588843No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC280300.1981038850270921Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACTCC277700.19626631777389753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT269710.19061933225350344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAA266670.18847079208053746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG262520.18553775204178458No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG255040.18025121240567096No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA242280.17123299773230066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG241240.17049797083102283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGTCTGAACT239870.16952971423991647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA232360.1642219719047275No Hit
AAGGCTAAATACCGGCACGAGACCGAGATCGGAAGAGCACACGTCTGAAC228050.16117585080423957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC221170.1563133651496324No Hit
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA220980.15617908138882203Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT212230.14999496082518643No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA211260.1493094068884177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA210540.14880054211060995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA210150.14852490702263077No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT200690.1418389892475459Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC200310.14157042172592516No Hit
AGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCC196470.13885647624428393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC184830.13062982900305897No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC183970.13002201829623308No Hit
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA180670.12768972139794765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC173800.12283430330969891No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG166780.11787287172607355No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC165670.11708837186028666No Hit
AAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGAA165380.11688341243589187Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG164680.11638868279080102Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC154670.10931404886600192No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC154060.10888292731813702No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC153640.10858608953108252No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA152740.10795000855882285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT152720.10793587342610599No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA149860.10591454944759195No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT149660.10577319812042313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)