FastQCFastQC Report
Thu 28 Sept 2023
SRR3131016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3131016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14080767
Total Bases718.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10413777.395740587142733No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT4583703.2552914198495015No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4464133.170374170668402No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG4194502.978886022331028No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3239062.300343440098114No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2393801.700049436227444Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2079951.4771567486345025TruSeq Adapter, Index 12 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1783551.2666568518604135No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1393370.9895554695280449No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1369670.972724000049145No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1278300.9078340689821798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA888750.6311801054587438No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA746430.5301060659550719No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT718690.5104054345903174Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA704910.5006190358806448No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC691810.4913155654091854No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC657150.4667004290320264No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT646850.45938548660026834No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA639070.45386021940424126No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC638170.4532210496771944Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC627660.4457569676424587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC623530.44282388878389933No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT599770.4259498079898631Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC588490.4179388807442095Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT566160.402080369627592No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG532490.3781683199501845No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA482770.3428577434737753No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC444460.31565041875914857No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG436080.309699038411757No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC419840.2981655757814897No Hit
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC387470.2751767712653721Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGTCTGAACTC354500.25176185359788994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT346670.24620107697258253No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC334300.23741604416861664No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA327510.23259386367234114Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC318870.22645783429269156No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT312970.22226772163760683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC306900.21795687692296875No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT304320.21612459037210116Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG291310.20688503687334645Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC282940.20094075841181092Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG279570.19854742287831337No Hit
ACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACTCC273320.19410874421826596Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT269540.19142423136466927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAA266640.18936468446640725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG259630.18438626248129808No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG252880.17959248952844684No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG242890.17249770555822705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGTCTGAACT241110.17123356987584554Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA239830.17032452848626783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA230370.16360614446642005No Hit
AAGGCTAAATACCGGCACGAGACCGAGATCGGAAGAGCACACGTCTGAAC228160.16203662769222726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA219200.1556733379651833Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC217760.15465066640190836No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA212240.15073042540935447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA211100.1499208104217618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT210320.14936686332498791No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA207040.14703744476419503No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT203980.1448642676922358Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC200860.14264847930514013No Hit
AGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCC196290.13940291746891345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC187450.13312485037214236No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC185030.131406193994972No Hit
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA182250.12943186972698292Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC173940.12353020258058385No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG164790.11703197702227443No Hit
AAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGAA164330.11670529027289493Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC163680.11624366769225No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG162530.11542695081880128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA155390.1103562043175631Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC154590.10978805344907704No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC154060.109411653498705No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT153140.10875827999994603No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT151000.10723847642674579No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC150640.10698280853592705No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA149200.10596013697265214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)