FastQCFastQC Report
Thu 28 Sept 2023
SRR3131025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3131025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49019994
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC31293216.383764551256371No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT14944023.0485560646947447No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC14641522.9868465508176114No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG14143232.885196191578481No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC10928462.229388277770903No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC8338251.7009896002843246Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG6783061.3837333395022446TruSeq Adapter, Index 12 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG6367401.2989393674752387No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4739520.9668544635072782No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT4384290.8943881143681902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA4152440.8470910869552534No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2989370.6098266760293769No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2524880.5150714624730472No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2432990.49632605014190745No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA2432280.49618121128289No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT2287490.4666442839629886Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2278360.46478177863506065No Hit
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC2278260.46476137879576235Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2277150.46453494057955214No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2252470.45950026024074997No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC2211050.45105064680342477Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2080950.42451045587643277No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC2024400.41297434675328604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1917870.3912423979488859Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1901660.38793558399864353No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1751160.35723382585481345No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1565020.31926156498509567No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG1517650.30959816110952604No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC1496840.30535295455156525No Hit
ACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTCTGAACTC1479400.3017952225779546Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1434290.2925928550705249No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1295260.26423095849420136No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA1239710.25289884776403687Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1238930.2527397290175107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1158480.23632805830208792No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1147410.2340697960917743Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1123380.2291677147084106No Hit
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG1085830.22150757505192678Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGTCTGAACTC1060740.21638925537200188Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC992770.20252348460099773No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAA978950.1997042268099829Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACTCC967550.19737864512998513Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT912000.1860465343998206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC900870.18377603228592806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG886770.1808996549448782No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG879190.17935334712607268No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA853620.1741371082175163No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG851550.17371483154404302No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG806240.16447166435801686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT794360.1620481634493876No Hit
CCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGTCTGAACT789870.16113221066489727Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC767320.15653204690314731No Hit
AAGGCTAAATACCGGCACGAGACCGAGATCGGAAGAGCACACGTCTGAAC762880.15562629403830608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA758690.1547715407717104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA745030.15198492272357275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT744650.1519074033342395Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCC740250.15100981040511754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA729930.14890454698954061Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA702020.14321095184140578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA701100.1430232733198621No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC684310.13959814030168996No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC645170.1316136432003643No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC642310.13103020779643507No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG623450.12718279810478963No Hit
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA621710.12682784090100052Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC618180.12610772657377314No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT599130.12222155718746111No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC596860.12175848083539136No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT587870.11992453528248087No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC577310.1177703122525882No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA564160.1150877333848715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGAA554590.11313546876403127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC548750.11194411814901488No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC528880.10789067008045738No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA516630.1053916897664247No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG502540.10251735240930467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)