FastQCFastQC Report
Wed 28 Jun 2023
SRR3143648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3143648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139362
Total Bases101.8 Mbp
Sequences flagged as poor quality0
Sequence length24-136
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1112643.544159609500274No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC927632.95483604630495No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC859462.7376900147227365No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC565931.8026911200428621No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGA494391.574810423264345No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA471041.5004322534323853No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGCC369931.178360443937335No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC349191.1122960652514746No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA322071.0259090859862607No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC291240.9277044189233354No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC257470.820134791718827No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT255970.8153567508302642No Hit
GGCCAAGGCGGGGGGGGATGCGTGCATTTATCAGA229970.7325373754285106No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG184110.5864567386621867No Hit
GGCCAAGGCGGGGGGGGGATGCGTGCATTTATCAGA160500.5112503750762097No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGCC156720.4992097120370317No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA142870.45509246783263607No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA134520.42849470688630364No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGA129890.4137464873436068No Hit
GGCCAAGGCGGGGGGGGATGCGTGCATTTATCAGAT122740.3909711591081245No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGA111620.355549949320913No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC108570.3458345995141688No Hit
GACTGCATAATTTGTGGTAGTGGGGG106960.34070616896044487No Hit
TCGACTGCATAATTTGTGGTAGTGGGG102500.3264994607184517No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGCC102380.3261172174473667No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG98790.31468177292073995No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT97600.31089119381581354No Hit
ATGTACACGGAGTCGACCCAACGCGA96560.3075784187997434No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG89860.28623650283082996No Hit
GGCCAAGGCGGGGGGGATGCGTGCATTTATCAGA88570.282127387666666No Hit
GGCCAAGGCGGGGGGGGGATGCGTGCATTTATCAGAT84870.27034155347487804No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT84390.2688125803905379No Hit
GGCCAAGGCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG77440.24667432427353075No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGC76930.2450497903714194No Hit
GGACCGGGGTCCGGTGCGGAGAGCCGTTCGT73630.23453810041658146No Hit
GGACCGGGGTCCGGTGCGGAGAGCCGTTCG70170.22351675276696348No Hit
GGCCAAGGCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGT69400.22106402511083462No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTCGT66880.21303691641804925No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC64910.20676175605107025No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCC63520.20233410482766878No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCC63340.2017607399210413No Hit
GGCCAAGGCGGGGGGGATGCGTGCATTTATCAGAT60670.19325582713939968No Hit
GGACCGGGGTCCGGTGCGGAGAGCCGTTC59290.188860029521922No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA56730.1807055064054416No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC56680.18054623837582287No Hit
GGCCAAGGCGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG54340.17309249458966502No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACA54060.1722005936238No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGC53210.16949303712028113No Hit
TACCCGTCGCCGCGCTCTCCCTCTCC51470.16395050968954838No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTCG49460.15754793489887436No Hit
CGGGTGGGGCCCAAGTCCTTCTGATCGAGGCC49200.15671974114485682No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATG48350.15401218464133795No Hit
CGACTGCATAATTTGTGGTAGTGGGGG47940.15270618679846415No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGC47790.1522283827096079No Hit
CTCCCGGGCTACGCCTGTCTGAGCGTCGCT47700.1519417002562941No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT45790.1458576615248576No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGCC45550.14509317498268756No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA45090.143627909110195No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTC44700.1423856184791687No Hit
GACTGCATAATTTGTGGTAGTGGGGGG44290.1410796206362949No Hit
GACTGCATAATTTGTGGTAGTGGGG43840.13964620836972608No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATG43580.13881801461570856No Hit
GACTGCATAATTTGTGGTAGTGGGGGA43300.1379261136498435No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC42990.1369386518662072No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC42340.13486816748116337No Hit
GGCCAAGGCGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG41480.13212875737172075No Hit
GGCCAAGGCGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGT40330.12846559269048932No Hit
GGCCAAGGCGGGGGGGGGGATGCGTGCATTTATCAGA39790.12674549797060677No Hit
GGCCAAGGCGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGT39620.126203986669903No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAT39190.12483428161518169No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCG39050.12438833113224915No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT37850.12056589842139899No Hit
GTTGAACCCATTCGTGATGGGGATCGGGGATTG35620.11346254430040242No Hit
CGACTGCATAATTTGTGGTAGTGGGG34150.10878006422961098No Hit
GGCCAAGGCGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT34090.10858894259406847No Hit
CCTCCCGGGCTACGCCTGTCTGAGCGTCGCT33230.10584953248462586No Hit
CGTGGGGGGGCCCAAAGTCCTTCTGATCGAGGCC32700.10416129137066704No Hit
GGCCAAGGCGGGGGGGGATGCGTGCATTTATCAG32000.10193153895600444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)