FastQCFastQC Report
Sun 2 Jul 2023
SRR3147100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3147100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61258516
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG10169181.6600434786895588No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG7458421.21753194282408No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC6556001.0702185472465575No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG6462081.0548868013714208No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG5759240.9401533657785637No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG5461220.8915038033242595No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC5319790.8684164010763825No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATTC5010710.8179613753620804No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT4690920.7657580213010711No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA4112990.6714152200487521No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC3973080.6485759465671679No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC3968770.6478723709206407No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT2865440.4677619026879463No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC2651130.4327773790667734No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG2384020.38917364566911805No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA2116910.3455699122714628No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTA1961870.3202607781096101No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC1873780.30588073664729326No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1814480.2962004499096909No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC1581320.25813880310127No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC1543420.2519519082048935No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT1451560.23695644210512704No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCCTGTAGGCACCATCAATTCG1375790.2245875495906561No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC1284470.20968023449996734No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATTC1222630.1995853115344812No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG1185820.193576351082354No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATTCGT1142200.18645570846019188No Hit
CGGCACGCAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG1119760.18279254430518688No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT1099860.17954401637806572No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT1091130.17811890839797687No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1042500.17018042030270533No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC1036500.1692009646462869No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT1028690.16792603986684887No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCCTGTAGGCACC1028620.16791461288419066No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA996420.16265820086141167No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC930260.15185806982330424No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC927590.15142221205619802No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC873990.14267240819219323No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT827120.13502122708947112No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG823210.1343829484867051No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGTCTGTAGGCACCATCAATTCG808860.13204041704177097No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT799080.13044390432180888No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC789320.1288506564540349No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA753160.1229478036980197No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA705500.11516766093386918No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGACTGTAGGCACCATCAATTCG699680.11421758894714328No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC679350.11089886669797878No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACCTGTAGGCACCATCAATT671560.10962720677072882No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA664410.10846002211349684No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA648350.10583834580648345No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCA620920.10136060103055713No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGATCTGTAGGCACCATCAATT617200.10075333852357768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)